猪脂肪细胞决定和分化因子1基因(ADD1)中一个新SNP的特性及其对肉质性状影响  

Characterization of a Novel SNP of Porcine Adipocyte Determination and Differentiation Factor-1 Gene (ADD1) and Its Effects on Meat Quality

作　　者：李长龙[1,2] 萨晓婴[2] 高会江[3] 孟和[1] 潘玉春[1] 

机构地区：[1]上海交通大学农业与生物学院动物科学系,上海200240 [2]浙江省医学科学院,浙江省实验动物中心,杭州310013 [3]中国农业科学院北京畜牧兽医研究所,北京10193

出　　处：《农业生物技术学报》2010年第4期725-731,共7页Journal of Agricultural Biotechnology

基　　金：上海市科技兴农重点攻关项目(沪农科攻字(2003)第1-1号);国家重点基础研究发展规划(973)项目(No.2004CB117502)共同资助

摘　　要：脂肪细胞决定和分化因子1(adipocyte determination and differentiation factor-1,ADD1)基因与人类和鼠的脂肪细胞分化具有确定的连锁关系。在脂肪细胞分化过程中,ADD1与转录因子PPARγ和C/EBP发挥着关键的作用。本研究克隆了保守DNA序列并用PCR-SSCP和直接测序法鉴定其多态性。同时,选择136头猪作为实验群体来探索其与肉质性状的关系。多态性分析发现:在第二外显子内一个导致错义突变的SNP,该SNP在实验群体内分布不平衡,未检测到AA基因型。相关分析结果显示,ADD1不同基因型间在肌内蛋白(MP)和肉色(MC)存在显著(P<0.05)的差异;肌内脂肪(IMF)存在极显著(P<0.01)的差异。说明,ADD1基因多态性有可能应用到提高肉质的育种实践中。Adipocyte determination and dfferentiation factor-1 gene （ADD1）,a gene associated with adipocyte differentiation in the research of human and rat,plays a role during adipocyte differentiation with PPARγ and C/EBP.In this paper,we cloned a part of the gene sequence from pig,screened it with PCR-SSCP （single strand conformation polymorphism）,and used sequencing to identify mutation.The result indicated that a SNP （single nucleotide polymorphism） happened in the conservative domain of the ADD1 gene,which led to a different amino-acid sequence and a new site for proteinase.To verity the relationship between this mutation and meat quality,we chose 136 pigs as the experimental population,all of which were butchered to determine meat quality.The result of polymorphism analysis showed that a novel SNP was found in second exon.The polymorphism was disequilibrium in population and AA genotype was not found in it.The relation analysis showed that intramuscular protein,intramuscular fat and meat color had obviously difference among different ADD1 genotypes of pigs.The ADD1 polymorphism can apply to pig breeding for improving meat quality.

关 键 词：猪 肉质 脂肪细胞决定和分化因子1基因(ADD1) PCR-SSCP SNP 

分 类 号：S852.2[农业科学—基础兽医学]