奶牛体外成熟卵母细胞与X分离冻精体外受精及其胚胎发育  

Fertilization of Cattle Oocytes with X-sorted Frozen-thawed Sperm and Embryo Development In vitro

作　　者：马保华[1] 刘闯[1] 徐红强[1] 赵晓娥[1] 

机构地区：[1]西北农林科技大学动物医学院,农业部动物生殖生理和胚胎工程重点开放实验室,杨凌712100

出　　处：《农业生物技术学报》2010年第4期753-759,共7页Journal of Agricultural Biotechnology

基　　金：863计划现代农业技术项目(No:2008AA101003;2008AA101010);陕西省重大科技攻关项目(No:2006KZ07-G1)共同资助

摘　　要：比较了X分离冻精和未分离冻精与牛卵母细胞进行体外受精后,受精卵的卵裂率和胚胎发育率。X分离冻精组受精卵的卵裂率(51.4%)和囊胚发育率(19.7%)显著低于未分离冻精组(67.9%,29.9%,P<0.05);微量上浮非离心法处理X分离冻精和未分离冻精后,其受精卵的卵裂率(50.7%,65.5%)高于上浮离心法处理组(48.3%,63.8%),但两种处理方法之间无统计学差异(P>0.05);同一种精子采用微量上浮非离心法或上浮离心法处理后,其卵裂胚的桑葚胚发育率和囊胚发育率无统计学差异(P>0.05);X分离冻精的受精卵与颗粒细胞和睾丸支持细胞共培养后,其卵裂率(50.0%,51.4%)与非共培养组(50.3%)无显著差异(P>0.05);两个共培养组的桑葚胚发育率(53.3%,54.6%)和囊胚发育率(21.1%,21.5%)均高于对照组(52.2%,20.4%),但无统计学差异(P>0.05)。The embryo developmental capacity after in vitro fertilization with the X-sorted frozen and unsorted frozen sperm were studied.The cleavage and blastocyst rate of embryos fertilized with X-sorted frozen sperm,were significantly lower than that of control group fertilized with unsorted frozen sperm （51.4% vs.67.9%,P 0.05;19.7% vs 29.9 %,P〈0.05）.After the two kinds of frozen sperm were treated with the micro epi-floating non centrifugation and the epi-floating,the cleavage rate of the former was better than that of the latter,but there were not statistically significant difference （50.7% and 65.5% vs.48.3% and 63.8%,P〈0.05）.Additionally,no statistical differences were observed in morula rate and blastocyst rate of the cleavaged zygotes fertilized with the X-sorted frozen sperm and unsorted frozen sperm.After co-culturing with sertoli cells and granular cells respectively,the cleavage rate （50.0% and 51.4%） of sexed zygotes co-cultured with feeder cells were not statistically significant difference with that of control group （50.3%,P〉0.05）.And the morular rate（53.3% and 54.6%） and blastular rate （21.1% and 21.5%） of sexed embryo co-cultured with feeder cells were higher than that of control group （52.2% and 20.4%）,but there were no statistically difference （P〉0.05）.

关 键 词：X分离冻精 体外受精 共培养 胚胎 奶牛 

分 类 号：S828.36[农业科学—畜牧学]