曲古菌素A对胃癌细胞SGC-7901增殖抑制的研究  

Effects of trichostatin A on growth of gastric carcinoma cell SGC-7901

作　　者：杨桐树[1] 赵婷[1] 李云龙[2] 郭宝良[2] 闫朝岐[2] 李晓林[2] 宋茂力[2] 邹小明[2] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院检验科,哈尔滨150081 [2]哈尔滨医科大学第二临床医学院普外科,哈尔滨150086

出　　处：《中国卫生检验杂志》2010年第8期1841-1842,1846,共3页Chinese Journal of Health Laboratory Technology

基　　金：黑龙江省卫生厅科研项目(2007-311);哈尔滨医科大学附属二院博士科研基金资助(NoBS2008-05)

摘　　要：目的:研究组蛋白去乙酰化酶抑制剂曲古菌素A(TSA)对胃癌细胞SGC-7901增殖活性的抑制作用。方法:采用不同浓度的TSA处理胃癌细胞SGC-7901,MTT法检测药物作用前后的细胞增殖情况。流式细胞仪检测TSA处理前后细胞周期的变化。结果:75 ng/ml TSA在48 h时就出现明显抑制胃癌细胞SGC-7901的增殖。抑制由12 h至72 h显著增加,细胞周期检测发现75 ng/ml TSA即可导致细胞G2期阻滞,抑制细胞增殖。结论:TSA可抑制体外胃癌细胞SGC-7901的生长,诱导SGC-7901细胞出现G2期阻滞。Objective：To investigate whether trichostatin A（TSA） possesses antitumor activity against human gastric carcinoma cell SGC-7901.Methods： Human gastric carcinoma cell SGC-7901 was treated with different concentrations of TSA.The growth of SGC-7901 cells were observed by MTT assay before and after TSA treatment.The cell cycle of SGC-7901 cells was analyzed by flow cytometry.Results： Trichostatin A significantly inhibited the proliferation of gastric carcinoma cells SGC-7901 cells at 75 ng/ml.The inhibition rate increased sharply from 12 h to 72 h.75 ng/ml TSA treatment could induce cell cycle arrest at the G2 phase,induce significant apoptosis as shown by flow cytometry.Conclusion： The results indicate that TSA is able to inhibit SGC-7901 cells growth in vitro,possibly through G2 cell cycle arrest.

关 键 词：胃肿瘤 曲古菌素A 凋亡 细胞周期 

分 类 号：R979.1[医药卫生—药品]