荧光毛细分析法测定胆固醇  

Determination of cholesterol by fluorescence capillary analysis method

作　　者：杨海朋[1] 王丙涛[2] 陈仕国[1] 李乔喜[1] 戈早川[1] 

机构地区：[1]深圳大学材料学院深圳市特种功能材料重点实验室,深圳518060 [2]深圳出入境检验检疫局食品检验检疫技术中心,深圳518067

出　　处：《中国卫生检验杂志》2010年第8期1862-1863,1866,共3页Chinese Journal of Health Laboratory Technology

基　　金：深圳大学科研启动基金资助项目(200818)

摘　　要：目的:建立采用荧光毛细分析法(FCA)测定液体中胆固醇含量的低成本方法。方法:样品溶液加入到含有一定量胆固醇氧化酶和过氧化物酶的对甲基酚溶液中反应一定时间,用毛细管吸取溶液放入毛细管固定座中,用荧光分光光度计测量荧光强度。结果:在优化的测试条件下,胆固醇浓度在1.0～10.0μmol/L范围内与荧光强度成线性关系,线性回归方程为:F=280.4+72.3c(n=5,r=0.99),样品加标回收率为88.2%～111.0%。结论:荧光毛细分析法灵敏度高,试剂消耗量减少99%,检测成本低,是检测液态样品中胆固醇的有效定性、定量方法。Objective：To develop a quick,low cost detection method for cholesterol by using fluorescence capillary analysis（FCA）.Methods： The mixed solution of p-cresol,cholesterol oxidase,horseradish peroxidase and cholesterol was inhaled into a capillary.The capillary was inserted into a capillary holder for fluorescence determination.Results： The linear range of the method was 1.0 to 10.0 μmol/L.The linear regression equation was F=280.4＋72.3c with r=0.99,and the recoveries were 88.2%～111.0%.Conclusion： The method is low cost and high sensitivity.It can be used to detect the cholesterol in liquid samples.

关 键 词：荧光毛细分析法 对甲基酚 胆固醇氧化酶 过氧化物酶 胆固醇 

分 类 号：O657.3[理学—分析化学]