液相色谱梯度洗脱法测定洗发类化妆品中的4种去屑剂  

Determination of 4 antidandruff agents in shampoo cosmetics by HPLC-gradient elution

作　　者：黄巧巧[1] 黄琴伟[1] 郑金琪[1] 

机构地区：[1]浙江省食品药品检验所,杭州310004

出　　处：《中国卫生检验杂志》2010年第8期1906-1907,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立液相色谱梯度洗脱法同时测定洗发类化妆品中的水杨酸、酮康唑、氯眯巴唑和吡罗克酮乙醇胺盐4种去屑剂。方法:采用C18柱,以乙腈-甲醇-0.01 mol/L的磷酸二氢钾溶液(含EDTA0.5 mol/L,磷酸调pH值至4.0)梯度洗脱分离各个组分,进样体积5μl,DAD检测器,检测波长230 nm。结果:水杨酸、酮康唑的线性范围为5μg/ml～500μg/ml,氯眯巴唑和吡罗克酮乙醇胺盐线性范围为10μg/ml～1000μg/ml,最低检出限分别为0.12μg/ml、0.19μg/ml、0.39μg/ml、0.98μg/ml。该方法精密度高,RSD在0.7%～1.5%之间,各组分加标回收率在98%～105%之间。结论:本方法具有操作简便、干扰小、快速等特点。Objective：A high performance liquid chromatography method with gradient elution was established for determination of antidangdruff agent salicylic acid,ketoconazole,climbazole and octopirox in shampoo.Methods：A C18 column was used as stationary phase,and mixture containing acetonitrile-methol-aqueous solution（10 mmol/L KH2PO4 and 5 mmol/L EDTANa2,pH is adjusted to 4.0 with H3PO4） eluted by gradient was used as mobile phase in the HPLC with DAD at the wavelength of 230 nm.Injection volume is 5 μl.Results：The linearity range of salicylic acid,ketoconazole,climbazole and octopirox is 5 μg/ml～500 μg/ml and 10 μg/ml～1000 μg/ml,the limits of detection are 0.12 μg/ml,0.19 μg/ml,0.39 μg/ml and 0.98 μg/ml respectively.The recovery varied from 98% to 105%,and the relative standard deviation is 0.7%～1.5%.Conclusion：The experimental results show that the method is simple,precise,little interference and quick.

关 键 词：去屑剂 液相色谱 洗发液 化妆品 梯度洗脱 

分 类 号：O657.72[理学—分析化学]