薄层层析-光度法测定马拉硫磷含量  

Determination O,O-Dimethyl-S-(1,2-dicarbethoxyethl) phosphorodithioate percentage in malathion by photometry Hyphenated with thin layer chromatography

作　　者：张锂[1] 韩国才[1] 

机构地区：[1]兰州工业高等专科学校基础学科部,兰州730050

出　　处：《中国卫生检验杂志》2010年第8期1908-1909,1912,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立薄层色谱-光度法测定了马拉硫磷中O,O-二甲基-S-(1,2-二乙酯基乙基)二硫代磷酸酯的方法。方法:采用薄层层析分离样品中的干扰物质,用乙醇淋洗,萃取分离杂质后,水解成O,O-二硫代磷酸,然后与Cu2+形成络合物,光度法测定样品含量。结果:回收率98%～105%。结论:本法操作简单、快速、结果准确可靠,应用于实际样品的测定,结果满意。Objective：To establish a method for detection of O,O–Dimethyl-S-（1,2-dicarbethoxyethl） phosphorodithioate Percentage in Malathion.Methods： The method of thin layer chromatograpty（TLC） was used to separate the main component,chromatographic band of the sample which contains the malathion,was taken down from the TCL plate.In HCl medium pH 3～4,O,O–Dimethyl-S-（1,2-dicarbethoxyethl） phosphorodithioate reacts with Cu2＋ to from a complex with its absorption maxima at 415 nm,was determined photometricallt.Results： The recovery of standard addition 98% to 105%.Conclusion： This method is simple,rapid,sensitive,results are accurate and reliable.The method has been applied to the sample analysis with satisfactory results.

关 键 词：薄层色谱 光度法 O O-二甲基-S-(1 2-二乙酯基乙基)二硫代磷酸酯 测定 

分 类 号：O657.37[理学—分析化学]