4723例生殖道沙眼衣原体DNA定量检测  

Quantitative detection of chlamydia trachomatis in 4723 cases patients

作　　者：欧阳耀灵[1] 卢亚祖[1] 高清萍[1] 范翠芳[2] 王嵛[3] 

机构地区：[1]湖北省荆州市中心医院检验医学部,湖北荆州434020 [2]湖北省荆州市中心医院妇产科,湖北荆州434020 [3]湖北省荆州市中心医院性病科,湖北荆州434020

出　　处：《中国卫生检验杂志》2010年第8期2009-2010,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:为了解CTDNA载量的流行病学特点。方法:FQ PCR方法用于定量检测2002年-2009年4723例患者的CTDNA。结果:2002年-2009年,CTDNA阳性率8.24%,其中男性阳性率8.45%(146/1727),女性阳性率8.11%(243/2996)(χ2=0.171,P=0.680);CTDNA阳性率无统计学变化(r=-0.595,P=0.120),男性(r=0.286,P=0.493)和女性(r=-0.667,P=0.071)CTDNA阳性率也无统计学差异。男性CT DNA载量(拷贝对数值)为5.21±1.79(r=-0.860,P=0.021),女性CTDNA载量为5.33±1.93(r=-0.786,P=0.021)。结论:2002年-2009年,CT DNA阳性率较稳定;男性与女性间CTDNA载量无统计学差异,男性和女性CTDNA载量逐年下降。Objective：To study the specificity of epydemiology of Chlamydia trachomatis（CT） DNA load.Methods：FQ-PCR was used to detect quantitatively CT DNA for the 4723 patients from 2002 to 2009.Results：The positive rates of CT DNA was 8.24%,8.45%（146/1727） in male and 8.11%（243/2996） in female（χ^2=0.171,P=0.680）.From 2002 to 2009,there was no change in statistics to the positive rates of CT DNA（r=-0.595,P=0.120）,and there were no ignificant difference in statistics in male（r=0.286,P=0.493）and in female（r=-0.667,P=0.071）;CT DNA load（logarithm of copies） ware 5.21±1.79 in male（r=-0.860,P=0.021）and 5.33±1.93 in female（r=-0.786,P=0.021）.Conclusion：From 2002 to 2009,the positive rates of CT DNA was identical.CT DNA load were no significant difference in statistics in male and in female.The CT DNA load went down in male and in female.

关 键 词：荧光定量PCR CTDNA阳性率 CTDNA载量 流行病学特点 

分 类 号：R374.1[医药卫生—病原生物学]