人参皂苷代谢产物衍生物对HepG2细胞凋亡及相关蛋白表达的影响  

Effect of a derivative of ginsenoside metabolite on apoptosis and relative protein expression in HepG2 cells

作　　者：杨春光[1] 弓晓杰[2] 孙长滨[3] 

机构地区：[1]大连大学医学院药理学教研室,辽宁大连116622 [2]大连大学医学院中药学教研室,辽宁大连116622 [3]大连大学医学院组织胚胎学教研室,辽宁大连116622

出　　处：《中国现代医学杂志》2010年第14期2100-2103,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No:30873155);国家自然科学基金资助项目(No:30973625)

摘　　要：目的探讨人参皂苷代谢产物衍生物对HepG2细胞凋亡的影响及机制。方法 MTT法检测不同浓度人参皂苷代谢产物衍生物对HepG2细胞增殖的作用;流式细胞仪分析肿瘤细胞凋亡周期及细胞凋亡率;Western Blot法测定药物对HepG2细胞凋亡相关蛋白表达的影响。结果人参皂苷代谢产物衍生物抑制肝癌HepG2细胞生长,呈浓度依赖关系。用50mg/L和100mg/L人参皂苷代谢产物衍生物处理细胞24h后,凋亡率与对照组比明显升高。流式细胞术分析显示S期和G2/M期细胞周期阻滞。Western Blot结果显示,人参皂苷代谢产物衍生物使细胞中Bax、Caspase-3蛋白表达增强,Bcl-2蛋白表达降低。结论人参皂苷代谢产物衍生物促进肝癌HepG2细胞凋亡,可能与下调Bcl-2蛋白表达,上调Bax、Caspase-3蛋白表达有关。【Objective】To investigate the effects and mechanisms of a derivative of ginsenoside metabolite on HepG2 cell apoptosis.【Methods】After treated with different concentrations of the derivative of ginsenoside metabolite,the proliferation was analyzed by MTT assay,cell cycle,and apoptosis rate of HepG2 cells were analyzed by flow cytometry（FCM）.Western Blot was used to detect protein expression of Bcl-2,Bax and Caspase-3.【Results】 The derivative of ginsenoside metabolite inhibited proliferation of HepG2 cells in a concentration-dependent manner.The S and G2/M phases cell cycle arrest were found by FCM and apoptosis in HepG2 cells was increased,after treated with 50 mg/L and 100 mg/L derivative of ginsenoside metabolite for 24 h.Western Blot showed that derivative of ginsenoside metabolite increased protein levels of Bax and Caspase-3 and decreased protein level of Bcl-2 in HepG2 cells.【Conclusions】The function of the derivative of ginsenoside metabolite on decreasing protein level of Bcl-2 and increasing protein levels of Bax and Caspase-3 might contribute to the apoptosis in HepG2 cells.

关 键 词：人参皂苷代谢产物衍生物 肝癌 蛋白表达 

分 类 号：R285.5[医药卫生—中药学] R735.7[医药卫生—中医学]