MG132对移植肝缺血再灌注损伤急性期的保护作用  

Effect of MG132 on transplanted liver subjected to ischemia/reperfusion injury during acute phase

作　　者：林艺雄[1] 周杰[1] 郑志鹏[1] 余晟[1] 赵宏峰[1] 任旋磊[1] 王峥[1] 

机构地区：[1]南方医科大学南方医院肝胆外科,广东广州510515

出　　处：《中国现代医学杂志》2010年第14期2136-2138,共3页China Journal of Modern Medicine

摘　　要：目的探讨蛋白酶体抑制剂MG132对大鼠移植肝缺血再灌注损伤急性期的保护作用。方法建立大鼠自体原位肝移植缺血再灌注损伤模型,经60min冷缺血和恢复血流灌注90min后,检测血清ALT、AST浓度和SOD、MDA的活性,并观察大鼠肝脏病理切片。结果 IRI+MG132组大鼠血清ALT、AST水平明显较IRI组大鼠下降(P<0.05),在MG132作用下,大鼠血清SOD活性升高,而MDA产生减少(P<0.05)。这些差异也可以从IRI+MG132组相对于IRI组Suzuki评分的改善得到证实。结论 MG132能够通过清除自由基和抑制脂质过氧化,保护大鼠移植肝急性期的缺血再灌注损伤。[Objeetive] The aim of the present study was to investigate whether the proteasome inhibitor MG132 protects rat liver against IRI during the acute phase. [Methods] An orthotopic autologous liver transplantation model was established. Levels of aspartate aminotransferase （AST）, and alanine aminotransferase （ALT）, as well as super-- oxidase dismutase （SOD）, and malonaldehyde（MDA） activities were measured in the plasma after sixty-rain cold ischemia followed by ninety-min reperfusion. Morphological changes of rat liver after reperfusion were observed. [Results] In IRI＋MG132 team, plasma ALT and AST levels were decreased than those in IRI groups （P 〈0.05）. Plasma SOD activities were increased and MDA were obviously decreased by MG132 （P 〈0.05）. These differentia were confirmed by improved Suzuki scores in IRI＋MG132 team. [Conclusion] MG132 exerts a protective effect during the early phase of IRI in rat liver through clearing free radical and inhibiting lipid peroxidation.

关 键 词：缺血再灌注损伤 蛋白酶体抑制剂 肝 

分 类 号：R617[医药卫生—外科学] R-332[医药卫生—临床医学]