丙泊酚对大鼠海马ERK1、ERK2磷酸化和ERK2 mRNA表达的影响  

Effects of propofol on ERK1,ERK2 phosphorylation and ERK2 mRNA expression in hippocampus in rats

作　　者：张英[1] 郑利民[1] 吴新民[2] 

机构地区：[1]北京大学深圳医院麻醉科,深圳市518036 [2]北京大学第一医院麻醉科

出　　处：《临床麻醉学杂志》2010年第7期607-609,共3页Journal of Clinical Anesthesiology

基　　金：深圳市科技计划项目(200903055)

摘　　要：目的探讨丙泊酚对大鼠海马ERK1、ERK2磷酸化和ERK2 mRNA表达水平的影响。方法 64只成年雄性SD大鼠随机分为丙泊酚组(P组,训练前15 min腹腔注射丙泊酚9 mg/kg,容量2 ml/kg)和生理盐水组(S组,注射等容量生理盐水)。记录100 s内大鼠不再钻入暗室所需的训练次数,记录给药后15 min(T0)、1 h(T1)、3 h(T2)、24 h(T3)时大鼠的记忆潜伏期。测定T0～T3时海马ERK1、ERK2、磷酸化ERK1(p-ERK1)、磷酸化ERK2(p-ERK2)及ERK2 mRNA的表达水平。结果与S组比较,P组大鼠不再钻入暗室所需的训练次数增加,T2和T3时记忆潜伏期缩短,p-ERK1降低,T0～T3时p-ERK2降低(P<0.01),T3时海马ERK2 mRNA表达水平显著降低(P<0.01)。结论丙泊酚可抑制大鼠海马ERK1、ERK2的磷酸化水平,并下调ERK2 mRNA的表达水平。Objective To investigate the effects of propofol on extracellular signal-regulated kinase 1 （ERK1） ,ERK2 phosphorylation and ERK2 rnRNA expression in the hippocampus in rats. Methods Sixty four male SD rats were randomly divided into 2 groups of P given propofol 9 mg/kg intraperitoneally 15 min before training, and S given normal saline as the control. All animals received a training for avoiding darkness. The times of trial to obtain learning criterion in 100 seconds were recorded. The memory retention and latency were recorded at 0 h（T0 ）, 1 h（Tl ）, 3 h（T2 ） and 24 h（T3 ） after the training. Hippocampus was also obtained at T0-T3 for determination of phospborylation ERKI（p-ERK1）, p-ERK2 and ERK2 mRNA expression. Results Compared with group S, the times of trial to obtain learning criterion were significantly increased,memory latency was shortened at T2 ,T3ERK1 phosphorylation was decreased at T2 ,T3ERK2 phosphorylation was decreased at T0-T3, and ERK2 mRNA expression was decreased at T3 in group P（P〈 0. 01） Conclusion Propofol can inhibit ERK1 and ERK2 phosphorylation and down-regulate ERK2 rnRNA expression in the hippocampus in rats.

关 键 词：丙泊酚 细胞外信号调节MAP激酶类 海马 

分 类 号：R965[医药卫生—药理学]