Clara细胞分泌蛋白对兔单肺通气肺损伤的保护作用  

Protective effects of Clara cell secretory protein on lung injury induced by one lung ventilation in rabbits

作　　者：刘流[1] 李李[1] 周海洋[1] 

机构地区：[1]中南大学附属湘雅二医院麻醉科,长沙市410011

出　　处：《临床麻醉学杂志》2010年第7期619-621,共3页Journal of Clinical Anesthesiology

基　　金：湖南省自然科学基金资助项目(07JJ3143)

摘　　要：目的观察Clara细胞分泌蛋白(CCSP)对兔单肺通气肺损伤的保护作用。方法 30只新西兰大白兔随机均分为三组:双肺通气3 h组(Ⅰ组);单肺通气2 h,随后双肺通气1 h组(Ⅱ组);单肺通气Clara细胞剥除组(Ⅲ组)(通气方法同Ⅱ组)。观察支气管Clara细胞百分率、血清和肺泡灌洗液(BALF)中CCSP含量、BALF中白细胞计数及中性粒细胞比值、血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6、IL-8水平、肺组织湿/干比值和肺组织病理学检查。结果 Clara细胞百分率、CCSP含量I组高于II组(P<0.05),II组高于III组(P<0.01);BALF中白细胞计数、中性粒细胞比值和血清中TNF-α、IL-6、IL-8水平、肺湿/干比值I组低于Ⅱ组(P<0.05),Ⅱ组低于Ⅲ组(P<0.01);病理学检查示肺损伤程度Ⅰ组轻于Ⅱ组,Ⅱ组轻于Ⅲ组。结论 Clara细胞分泌蛋白可减轻单肺通气所致肺损伤。Objective To observe the protective effects of Clara cell secretory protein （CCSP） on pulmonary injury induced by one lung ventilation in rabbits. Methods Thirty New Zealand rabbits were equally randomized into group Ⅰ（two lung ventilation for 3 h）, group Ⅱ（one lung ventilation for 2 h, followed by two lung ventilation for 1 h）, and group Ⅲ（Clara cells depletion and one lung ventilation with the same method in group Ⅱ）. The percentage of Clara cells in bronchi, the content of CCSP in serum and bronchial alveolar lavage fluid（BALF）, the number of leukocytes, the percentage of neutrophils in BALF, the level of TNF-α, IL-6, IL-8 in serum, the pulmonary wet/dry weight ratio, and the pulmonary pathological examination were observed. Results The percentage of Clara cells and the content of CCSP were in an order of group Ⅰ〈group Ⅱ（P〈0.05）〈group Ⅲ（P〈0. 01）. The number of leukocytes, the percentage of neutrophils, the level of TNF-α, IL-6, IL-8, and the pulmonary wet/dry weight ratio were in an order of group Ⅰ 〈group Ⅱ 〈group Ⅲ（P〈0. 05）. The pulmonary pathological examination showed that the degree of lung injury was in an order of group Ⅰ 〈group Ⅱ 〈group Ⅲ. Conclusion CCSP can mitigate the lung injury induced by one lung ventialtion.

关 键 词：单肺通气 肺损伤 Clara细胞分沁蛋白 

分 类 号：R563[医药卫生—呼吸系统]