HPLC测定比较曼地亚红豆杉和南方红豆杉不同部位的紫杉醇含量  

Taxol Content Comparison in Different Parts of Taxus madia and Taxus chinensis var. mairei by HPLC

作　　者：董权锋[1] 刘晶晶[1] 于荣敏[1] 

机构地区：[1]暨南大学药学院,广东广州510632

出　　处：《中药材》2010年第7期1048-1051,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:分析和比较曼地亚红豆杉和南方红豆杉不同部位的抗癌活性成分紫杉醇的含量。方法:利用85%乙醇提取和CH2Cl2萃取得到相关成分;通过高效液相色谱法进行了方法学研究并对紫杉醇含量进行了测定和比较。结果:得到了紫杉醇等相关成分,建立了一个简单而可靠的方法学研究,以此为基础,检测分析并比较了两种植物不同部位中紫杉醇的含量,其中以南方红豆杉的树叶中紫杉醇含量最高,达到了5.18×10-5(w/w)。结论:该方法能准确简便地提取红豆杉植物中的紫杉醇并分析其含量,可为合理开发红豆杉提供一定的科学依据。Objective ：To analysis and compare the taxol content in different parts of Taxus madia and Taxus chinensis var. mairei by High Performance Liquid Chromatography（HPLC）. Methods ：85% EtOH and CH2Cl2 were used for the extraction of taxol. By HPLC,the methodology study and taxol content investigation were performed. Results ：The taxol was extracted successfully. One simple and reliable methodology was built up. Basing on these,the taxol content in these two Taxus spp. were analysed and compared,among of which the leaf of Taxus chinensis var. mairei has the highest taxol content（5.18×10-5,w/w）. Conclusion ：Taxol and its content in the original plants can be simply and reliably extracted and investigated by these methods,which also can provided the scientific basis for the rational development of Taxus spp.

关 键 词：紫杉醇 曼地亚红豆杉 南方红豆杉 高效液相色谱法 含量比较 

分 类 号：R284.1[医药卫生—中药学]