野菊花对实验性心室重构的影响  

Effect of Chrysanthemum indicum on Ventricular Remodeling in Rats

作　　者：吴琦[1] 陈长勋[1] 顾伟梁[1] 高建平[1] 王樱[1] 吕见[1] 

机构地区：[1]上海中医药大学药理教研室,上海201203

出　　处：《中药材》2010年第7期1112-1115,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30572379);上海市教委项目(08CZ09)

摘　　要：目的:研究野菊花水提液对异丙肾上腺素(ISO)诱发小鼠心室重构及L-甲状腺素(L-Thy)诱发大鼠实验性心室重构的影响。方法:小鼠每天scISO2mg/kg,连续7d;大鼠每天ipL-Thy0.25mg/kg,连续9d,制备心室重构动物模型。小鼠经药物干预7d后,称重法测定心脏指数(HW/BW)及左心室指数(LVW/BW);放射免疫法测定心肌血管紧张素Ⅱ(AngⅡ)含量;大鼠经药物干预9d后,称重法测定心脏指数(HW/BW)及左心室指数(LVW/BW);放射免疫法测定心肌血管紧张素Ⅱ(AngⅡ)、血清醛固酮(ALD)和肿瘤坏死因子(TNF-α)的水平;心肌样本碱水法测定羟脯氨酸(Hyp)的含量。结果:与正常对照组比较,给药7d时ISO诱发心室重构模型小鼠的HW/BW、LVW/BW及AngⅡ含量升高(P<0.05),野菊花水提液能显著改善模型小鼠心脏指数,降低心肌AngⅡ含量(P<0.05)。与正常对照组比较,给药9d时,L-Thy诱发心室重构模型大鼠HW/BW、LVW/BW,AngⅡ、ALD、TNF-α及Hyp含量均显著升高(P<0.05),野菊花水提液能显著改善大鼠心脏指数,降低血清ALD、TNF-α和心肌AngⅡ、Hyp水平(P<0.05)。结论:野菊花具有抑制实验动物心室重构的作用,其作用机制可能与抑制交感神经系统过度兴奋,降低AngⅡ、ALD和TNF-α等神经内分泌因子过度表达有关。Objective ：To explore the effects and mechanism of Chrysanthemum indicum on experimental ventricular remodeling induced by isoprenaline （ISO） and L-thyroxine （L-Thy）. Methods ：The ventricular remodeling of mice were induced by subcutaneous injection of ISO with the dosage of 2 mg/kg daily for 7 d and the rats with L-Thy intraperitoneally with the dosage of 0.25 mg/kg daily for 9 d. After 7 days＇ treatment,the cardiac index and the AngⅡ content in myocardium of mice were measured. After 9 days＇ treatment,the ratios of LVW/BW,HW/BW of rats were calculated,the AngⅡ content in heart tissue and the ALD,TNF-α concentration in serun were determined by radioimmunoassay,the Hydroxy proline（Hyp） content in heart tissue were measured by hydrolysis method. Results ：After 7～9 days of treatment,Chrysanthemum indicum significantly reduced the left ventricular weight index and heart weight index in mice and rats with myocardial hypertrophy,decreased the content of AngⅡ in ventricular tissue in mice and rats,and reduced the ALD,TNF-α concentration in serum and the Hyp content in ventricular tissue in rats（P〈0.05）. Conclusion ：Chrysanthemum indicum can significantly attenuate the experimental ventricular remodeling;the mechanism may be related with restricting the activity of the sympathetic nervous system and decreasing the levels of AngⅡ,ALD and TNF-α.

关 键 词：野菊花 异丙肾上腺素(ISO) L-甲状腺素(L-Thy) 心室重构 

分 类 号：R285.5[医药卫生—中药学]