大孔吸附树脂纯化黄芪总黄酮和总皂苷的研究  

Study on Purification of Total Flavonoids and Saponins of Astragalus with Macroporous Resin

作　　者：王治平[1] 高英[2] 李卫民[1] 刘杰[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学新药研究开发中心,广东广州510006

出　　处：《中药材》2010年第7期1163-1166,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:系统研究影响大孔吸附树脂吸附分离的因素,筛选纯化黄芪总黄酮和总皂苷的最佳吸附树脂。方法:以黄芪总黄酮和总皂苷含量为考察指标,根据树脂的吸附和解吸能力,从9种树脂中筛选出XDA-5、D101、AB-8,对优选的XDA-5绘制吸附等温线、漏出曲线,并优化纯化工艺条件。结果:XDA-5型树脂的纯化工艺条件为:取适当浓度的黄芪提取液,上样吸附速度为2BV/h,饱和吸附后(总黄酮的饱和吸附量为58.83mg/mL)先以4BV水洗脱,再分别以4BV30%、50%及70%乙醇解吸。所得产物中总黄酮和总皂苷含量分别为28.75%、44.38%。结论:XDA-5可纯化黄芪总黄酮和总皂苷,方法简便可行,纯化效果好。Objective ：Through systematic study on many factors of influence of macroporous resin adsorption and separation,screening the best macroporous resin for purification of total flavonoids and saponins of Astragalus.Methods ：The adsorption and desorption properties of total flavonoids and saponins on macroporous resins including D101,AB-8,XAD-1,HP-20,SP825,LSA-40,LX-18,XDA-5 and ADS-7 were compared. According to the results,XDA-5,D101 and AB-8 offered higher adsorption and desorption capacities and higher adsorption speed for total flavonoids and saponins than other resins.Column packed with XDA-5 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process. Results ：XDA-5 type macroporous resin owned optimum adsorption and elution parameters. The dynamic adsorption ratio of total flavonoids of Astragalus was 58.83 mg/mL after eluted with 4BV of distilled water and 4BV of 30%、50%、70% ethanol ,respectively. Contents of total flavonoids and saponins of Astragalus were 28.75%、44.38%,respectively. Conclusion ：It is a reliable and accurate method for development and industrial production of compound drug containing Astragalus.

关 键 词：黄芪总黄酮 黄芪总皂苷 大孔吸附树脂 吸附动力学曲线 吸附等温线 漏出曲线 

分 类 号：R284.2[医药卫生—中药学]