HPLC-MS对仙灵骨葆胶囊含量测定方法的探讨  

Study on the Content Determination for Xianlin Gubao Capsules by HPLC-MS

作　　者：高国英 吴越 倪艳娜 

机构地区：[1]江苏省无锡药品检验所,无锡214021

出　　处：《中国药品标准》2010年第4期298-301,共4页Drug Standards of China

摘　　要：目的：选择合适的流动相对《国家中成药标准汇编》（骨伤科分册）收载的仙灵骨葆胶囊中淫羊霍苷进行含量测定。方法：对现行质量标准中的流动相和改进的流动相方法进行了比较。色谱条件为LichrospherC18（4．6mm×250mm，3．5μm），乙腈-水（30：70）为流动相，检测波长270nm，流速为1．0mL·min^-1。结果：改进的方法能有效分离供试品中的淫羊霍苷，且淫羊藿苷的线性范围为51.25～256．25μg·mL^-1，加样回收率为100，4％。结论：为准确测定仙灵骨葆胶囊中淫羊霍苷含量提供了参考依据。Objective：To establish a new of assay method of Xianlin Gubao capsules by changing mobile phase. Methods：Compare existing quality standard with the revised method by the chromatogram peak and result. The C18 column was applied,using acetonitrile- water （30： 70） as mobile phase with flow rate of 1.0 mL· min^-1. The detection wavelength was 270 nm. Results：The revised method can improve the resolution. The linear range of icariin was 51.25 -256. 25 g · mL^-1 and correlation coefficient was 0. 999 9. The average recovery of sample was 100. 4%. Conclusion： The method was accurate for evaluating the quality of icariin in Xianlin Gubao capsules.

关 键 词：流动相 HPLC-MS 仙灵骨葆胶囊 淫羊霍苷 

分 类 号：R286.0[医药卫生—中药学]