慢生型大豆根瘤菌菌株GX201的脯氨酸脱氢酶基因(putA)的分子克隆  

Molecular Cloning of the Proline Dehydrogenase Gene (putA) from Bradyrhizobium japonicum Strain GX201

作　　者：何曙光[1,2] 武波[1,2] 柏学亮 马庆生 

机构地区：[1]广西师范大学生物系 [2]广西大学农业分子遗传农业部重点开放室

出　　处：《广西农业生物科学》1999年第1期24-28,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：国家高技术"863"计划资助;广西自然科学基金

摘　　要：根据已报道的脯氨酸脱氢酶基因（ｐｕｔＡ）序列，通过ＰＣＲ方法，从慢生型大豆根瘤菌菌株ＧＸ２０１的总ＤＮＡ中扩增到—ＰＣＲ产物。序列分析表明，该ＰＣＲ产物的长度为４１８ｂｐ，与已报道的ｐｕｔＡ基因具有９３．５％的同源性。以广谱寄主范围质粒ｐＬＡＦＲ３为载体，在大肠杆菌ＤＨ５α中构建了ＧＸ２０１的基因文库，并以该ＰＣＲ产物为探针，从基因文库中筛选到一重组质粒ｐＧＸＮ３００。A PCR product was amplified from the total DNA of the Bradyrhizobium japonicum strain GX201 according to reported sequence of the proline dehydrogenase gene (putA) DNA sequence shows that the PCR product consist of 418bp and share 93 5% homology with reported putA gene A gene library of GX201 was constructed in E coli strain DH5α by using broad host range cosmid vector pLAFR3,and a recombinant plasmid was screened from the gene library by using PCR product as probe

关 键 词：慢生型大豆根瘤菌 脯氨酸脱氢酶基因 克隆 

分 类 号：Q939.114[生物学—微生物学] Q78