慢生型大豆根瘤菌nfe基因的分子克隆  

Molecular Cloning of nfe Gene of Bradyrhizobium japonicum

作　　者：周刚林[1,2] 武波[1,2] 唐东阶[1,2] 柏学亮[1,2] 马庆生[1,2] 

机构地区：[1]广西大学农学院 [2]广西大学农业分子遗传农业部重点开放实验室

出　　处：《广西农业生物科学》1999年第1期29-32,共4页Journal of Guangxi Agricultural and Biological Science

基　　金：国家高技术"863"计划资助;广西自然科学基金

摘　　要：ｎｆｅＣ基因是从慢生型大豆根瘤菌中克隆到的，与竞争结瘤有关的基因。本研究从慢生型大豆根瘤菌菌株ＧＸ２０１的ｐＬＡＦＲ３为载体的基因文库中，筛选出与ｎｆｅ同源基因克隆。以转座子Ｔｎ５ｇｕｓＡ５诱变获得了ｇｕｓ基因表达的Ｔｎ５ｇｕｓＡ５插入突变质粒。It had been shown that nfeC gene was essential for nodulation competitiveness of Bradyrhizobium japonicum.In this work, a genomic library of DNA of a B.japonicum strain GX201 was constructed in pLAFR3,a broad host range consmid vector within many Gramnegative hosts. The genomic libraries were screened for the clone showing structural homology to nfeC gene.The recombinant plasmids were constructed by using Tn5gusA5 transposon to mutagenize the clone.

关 键 词：大豆根瘤菌 结瘤基因 分子克隆 nfe基因 

分 类 号：Q939.114[生物学—微生物学] Q78