气相色谱法测定右旋棉酚衍生物的含量  

Content Determination of (+)-Gossypol Derivates by GC

作　　者：关丽[1] 陈欢[1] 唐辉[1] 张欢[1] 杨四涛[1] 

机构地区：[1]新疆特种植物药资源教育部重点实验室,石河子大学药学院,石河子市832002

出　　处：《中国药房》2010年第33期3141-3143,共3页China Pharmacy

基　　金：教育部重点科技研究项目(205185);新疆生产建设兵团博士资金项目(2009JC14);石河子大学高层次人才科研启动资金专项(RC-ZX200762)

摘　　要：目的:建立右旋棉酚衍生物含量测定的气相色谱法。方法:采用气相色谱内标法,色谱柱为5%Phenyl Methyl Siloxane毛细管柱,氢火焰离子化检测器,载气为氮气,程序升温:初始温度为200℃,保持2min,然后以20℃·min-1的速度上升到240℃,保持3min;甲硝唑为内标,以内标法计算右旋棉酚衍生物的含量。结果:右旋棉酚衍生物检测浓度线性范围为80.06～480.36μg·mL-(1R2=0.9994),平均加样回收率为96.67%,RSD=1.4%。结论:该法灵敏、简便、快速,适用于右旋棉酚衍生物的含量测定。OBJECTIVE： To establish GC method for the content determination of （＋）-gossypol derivates. METHODS： GC internal standard method was developed for the content determination of （＋）-gossypol derivates. The GC was equipped with a flame-ionization detector. A capillary column of 5% Phenyl Methyl Siloxane was used with nitrogen as carrier. Programmed temperature was as follows： the initial temperature was 200 ℃ and maintained for 2 min, then raised to 240 ℃ with a rate of 20 ℃·min-1 and maintained for 3 min; metronidazole was used as an internal standard, and internal standard method was used to calculate the content of （＋）-gossypol derivates. RESULTS： The linear range of （＋）-gossypol derivates was 80.06～480.36 μg·mL-1（R2=0.999 4） with an average recovery of 96.67% （RSD=1.4%）. CONCLUSION： The method is sensitive, simple, rapid and suitable for the content determination of （＋）-gossypol derivates.

关 键 词：右旋棉酚衍生物 气相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学] R977.4