高效液相色谱法同时检测化妆品中7种性激素的条件选择及简化前处理  

Simplified Pretreatment and Simultaneous Determination of Seven Sex Hormones in Cosmetics via High Performance Liquid Chromatography

作　　者：袁金华[1] 黄薇[1] 查河霞[1] 宋黎军[1] 

机构地区：[1]南京市疾病预防控制中心,江苏南京210003

出　　处：《环境与职业医学》2010年第8期509-511,514,共4页Journal of Environmental and Occupational Medicine

基　　金：南京市医学重点科技发展项目(编号:ZKX06030);江苏省预防医学科研课题(编号:Y2006026)

摘　　要：[目的]简化预处理步骤，提高化妆品中7种性激素检测的准确度、精密度。[方法]利用乙醇溶解超声分散离心过滤，选择合适的色谱条件，实现7种性激素的完全分离和同时检测。[结果]实验中的预处理步骤能满足测定需要，确定检测波长为230nm，选择流动相乙腈：水（40：60，体积比），保留时间〈30min，7种性激素组分完全基线分离，能避开杂质峰的干扰。7种性激素的工作曲线在0．1～50．0mg／L的范围内呈良好的线性关系，回归方程的相关系数〉0．999。回收率为84％。104％，相对标准偏差为1．9％-8.3％，最低检出限0．01～0．06mg/L。[结论]按照本实验预处理方法进行测定，可实现7种性激素组分完全基线分离和同时测定。准确度、精密度和检出限均满足实验测定要求。[ Objective] To develop a simplified pretreatment method for simultaneous concentration determination of seven hormones in cosmetics via high performance liquid chromatography（ HPLC ）. [ Methods ] After a simplified pretreatment of the samples, the proper HPLC conditions were optimized. [ Results ] This simplified pretreatment was good enough for the measurement. An XTerra TMRP18 column was employed and a mixture of aeetonitrile ： aqueous（ 40 ： 60 ）was used as mobile phase. The measuring wavelength was 230 nm. The proper HLPC conditions were investigated for the determination of seven hormones in the linear range 0.1-50.0 mg/L （ correlation coefficients greater than 0.999, and the recoveries for the seven sex hormones ranged from 84%-104% with relative standard deviations of 1.9%-8.3%. The lowest detection limits were 0.01-0.06mg/L. [ Conclusion ] The method is suitable for sitmultaneous determination of the seven sex hormones in cosmetics with simplicity and accuracy.

关 键 词：性激素 化妆品 高效液相色谱法 前处理 

分 类 号：R115[医药卫生—公共卫生与预防医学]