荧光猝灭法测定新型除草剂ZJ0777  

Determination of New Herbicide ZJ0777 by the Fluorescence Quenching Method

作　　者：张培敏[1] 习玲玲[1] 马易敏[1] 吴军[1] 

机构地区：[1]浙江大学化学系,杭州310028

出　　处：《现代科学仪器》2010年第4期82-84,共3页Modern Scientific Instruments

基　　金：国家自然科学基金(J0830413);浙江省科技计划项目(2007F70075);浙江省自然科学基金(Y407118)资助

摘　　要：本文研究了除草剂ZJ0777[C19H18BrN3O3]的荧光猝灭光度法的测定方法。在1.5%乙酸-乙酸乙酯介质中,水杨酸与嘧啶苄胺类除草剂ZJ0777形成离子缔合物,导致水杨酸溶液荧光猝灭。当分别于最大激发/最大发射(λex/λem)305nm/442nm进行测量时,荧光猝灭值(ΔF)与ZJ0777浓度在0.2～10mg/L范围呈良好的线性关系,该方法灵敏度高,检出限为0.1mg/L。考察了共存物质的影响,表明该方法有良好的选择性。据此发展了一种高灵敏、简便快速测定微量除草剂ZJ0777的新荧光猝灭分光光度法。用于水样中除草剂ZJ0777的含量测定,结果满意。The spectrofluorimetric determination of ZJO777 using salicylic acid as an emission reagent has been investigated by measuring the decrease of fluorescence intensity of salicylic acid due to forming an ion association complex of ZJ0777-salicylic acid. An emission peak of salicylic acid, which is decreased linearly by addition ZJ0777, occurs at 442nm in 1.5% CH3COOH-CH3COOC2H5 solution with excitation at 305nm. The linear range for ZJ0777 is from 0.2 to 10mg/L, and its detection limit is 0.1 mg/L. The influence of coexisting substance was also inspected, and the result showed that this method has a better selectivity. Therefore,a new fluorescence method, which is high sensitive, simple and rapid, was developed to determinate ZJ0777 at trace amounts. The method could be applied to the determination of Z J0777 in water sample with satisfactory results.

关 键 词：除草剂 ZJ0777 水杨酸 荧光猝灭 

分 类 号：TQ457.2[化学工程—农药化工]