葛根素对高血糖模型大鼠降糖作用的机制研究  

Antihyperglycemic Effect of Puerarin in Experimental Diabetes Mellitus Rats

作　　者：陈秀芳[1] 董敏[2] 雷康福[1] 管瑜[2] 金丽琴[1] 

机构地区：[1]温州医学院生物化学教研室,浙江温州325035 [2]温州医学院生物学实验教学中心,浙江温州325035

出　　处：《中国药学杂志》2010年第16期1242-1246,共5页Chinese Pharmaceutical Journal

基　　金：温州市科技局科研基金资助项目(Y2005A002)

摘　　要：目的探讨葛根素对糖尿病大鼠的降血糖作用及其可能机制。方法 SD大鼠随机分成6组:正常对照组,糖尿病模型组,葛根素高(160 mg.kg-1)、中(120 mg.kg-1)、低(80 mg.kg-1)剂量治疗组,氨基胍(AG,100 mg.kg-1)治疗组;各组大鼠每天腹腔注射相应药物一次,正常对照组及糖尿病模型组腹腔注射等体积丙二醇。治疗12周后,用生化、免疫学方法测定血糖、胰岛素及血清、脑垂体、胰腺组织β-内啡肽(β-endorphin,β-EP)含量,采用RT-PCR方法检测脂肪、骨骼肌组织过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)、葡萄糖转运体4(glucose transporter-4,GLUT-4)mRNA的表达水平。结果糖尿病模型组大鼠血糖含量明显高于正常对照组(P<0.01),而胰岛素和血清、脑垂体、胰腺组织β-EP含量及脂肪、骨骼肌组织PPAR-γ、GLUT-4 mRNA表达明显低于正常对照组(P<0.05或P<0.01)。经葛根素治疗后,血糖水平明显降低(P<0.01),而胰岛素和血清、脑垂体、胰腺β-EP含量及脂肪、骨骼肌PPAR-γ、GLUT-4 mRNA表达明显升高(P<0.05或P<0.01)。结论葛根素对糖尿病大鼠血糖有降低作用,其机制可能与增加脑垂体、胰腺组织β-EP合成,增加胰岛素分泌,上调脂肪、骨骼肌组织GLUT-4基因的表达,促进葡萄糖的摄取利用有关。OBJECTIVE To investigate the anti-hyperglycemic effect of puerarin and its possible mechanisms in streptozotocin-induced diabetic rats.METHODS The diabetes mellitus rat model made by intraperitoneal injection of streptozotocin（60 mg·kg^-1）were randomly divided into diabetes mellitus model group,high dose puerarin（160 mg·kg^-1）,middle dose puerarin（120 mg·kg^-1）,low dose puerarin（80 mg·kg^-1） treatment groups and aminoguanidine（100 mg·kg^-1） treatment group.12 rats were in normal control group.Puerarin and aminoguanidine were intraperitoneally injected to rats once a day for 12 weeks.The animals of normal control group and diabetes mellitus model group were given equal volum propylene glycol.The blood glucose concentration was measured by glucose oxidase method.The serum insulin level was determined by 125I-insulin radioimmunoassay kit.The β-endorphin（β-EP） content in serum,pancreas and pituitary was detected by enzyme linked immunosorbent assay（ELISA）.The mRNA expression of peroxisome proliferators activated receptor γ（PPAR-γ） and glucose transporter-4（GLUT-4） in fatty tissues and skeletal muscles were determined by reverse transcription polymerase chain reaction（RT-PCR）.RESULTS The blood glucose concentration increased obviously（P〈0.01） but the serum insulin level and the content of β-EP in serum,pancreas and pituitary tissues as well as PPAR-γ and GLUT-4 mRNA expression in fatty tissue and skeletal muscle decreased significantly（P〈0.05 or P〈0.01,respectively） in diabetes mellitus model group,compared with those in normal control group.In puerarin treatment groups,the above changes were reversed,significant differences of those were found as compared to those in diabetes mellitus model group（P〈0.05 or P〈0.01,respectively）.CONCLUSION Puerarin can significantly attenuate hyperglycemia in diabetic rats,which is probably due to inducing the synthesis of β-EP in pancreas and pituitary tissues and stimulating of insulin secretion,and up-regulati

关 键 词：葛根素 糖尿病 Β-内啡肽 葡萄糖转运体4 大鼠 

分 类 号：R285.5[医药卫生—中药学]