HPLC荧光法测定高三尖杉酯碱在大鼠体内的分布  

Study on Tissue Distribution of Homoharringtonine in Rats by HPLC Using Fluorescence Detection

作　　者：王向军[1,2] 张旭文[1] 高渊[1] 骆快燕[1] 刘洋[1] 郭殿武[1] 

机构地区：[1]杭州民生药业集团有限公司产品开发部,杭州310011 [2]浙江九洲药业股份有限公司,浙江台州318000

出　　处：《中国现代应用药学》2010年第8期735-738,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的研究高三尖杉酯碱注射液在大鼠体内的组织分布,为该药进行临床研究及合理应用提供依据。方法生物样品在碱性条件下通过氯仿提取,用KromasilC18柱(4.6mm×150mm,5μm),乙腈-10mmol·L-1KH2PO4缓冲液(含0.2%三乙胺,用H3PO4调pH至2.5)(25:75)为流动相,荧光检测波长为λex280nm,λem320nm。结果绝对回收率为91.27%～102.4%,最低检测限为0.5ng·mL-1。SD大鼠单次尾静脉注射高三尖杉酯碱注射液0.5,1,2h后主要效应器官的浓度分布特点是:C骨髓>C心,主要消除器官的浓度分布特点是C肝>C肺>C肾。结论本法准确,灵敏度高,可用于高三尖杉酯碱的体内过程研究。高三尖杉酯碱在骨髓中有较高浓度分布,应引起重视。OBJECTIVE To establish an HPLC method for the study of tissue distribution of homoharringtonine in rats.METHODS The biological samples were extracted with chloroform in alkalinity condition.The Kromasil C18 column（4.6 mm×150 mm,5 μm） was used as analytical column with a mobile phase consisted of acetonitrile-10 mmol·L-1 phosphate buffer solution（0.2% triethylamine,pH 2.5）（25：75） at a flow rate of 1.0 mL·min-1 with fluorescence detector set at λex 280 nm and λem 320 nm.RESULTS The recovery of the method was 91.3%-102.4%,the LOD was 0.5 ng·mL-1.In main effect tissues,the homoharringtonine concentration was followed as in order Cbm Ccoeur,and that was CliverClungCkidney in eliminated tissues.CONCLUSION The method has a good selectivity and reproducibility and can be used for the investigation of homoharringtonine in biosamples.

关 键 词：高三尖杉酯碱 高效液相色谱法 荧光检测 组织分布 

分 类 号：R917.101[医药卫生—药物分析学] R965[医药卫生—药学]