LC-MS/MS快速筛查中毒药物及典型病例分析  

LC-MS/MS FAST SCREENING OF DRUG POISONS TYPICAL APPLICATIONS

作　　者：李鹏飞[1] 吴诚[1] 马萍[1] 刘丽宏[1] 王玲[1] 王彦峰[1] 高文静[1] 

机构地区：[1]第二炮兵总医院药学部,北京100088

出　　处：《北京师范大学学报（自然科学版）》2010年第4期506-509,共4页Journal of Beijing Normal University(Natural Science)

摘　　要：建立血液中常见中毒药物的快速、高效LC-MS/MS筛查方法.采用沉淀蛋白的前处理方法,通过相关信息扫描模式,运用多反应监测作筛查,再依赖3个不同能量的增强型二级碎片离子扫描,最终在数据库中筛选中毒药物.结果表明:优化LC-MS/MS快速筛查方法,节省了检测时间,已成功地进行毒物检测分析,为临床抢救赢得了时间、提供了有力的依据.本方法可用于中毒患者的毒物药物定性分析.The aim of this work was to establish qualitative screen for common drug poisons in blood by LC-MS/MS.Samples were treated by acetonitrile precipitation to remove protein.The scan was carried out with multiple reaction monitoring and enhanced product ion scan in a information-dependent acquisition mode.Drug was identified by library search with a newly developed MS/MS library based on EPI at three different collision energies in positive mode.The method has been successfully used in drug poison detection by LC-MS/MS,which saved time and provided evidence for the clinic.The method was simple,rapid and precise for qualitative analysis of common drug poison.

关 键 词：高效液相色谱-串联质谱 药物中毒 快速筛查 

分 类 号：O657.63[理学—分析化学]