大鼠肾脏糖胺聚糖的种类及二糖组成分析  

Sorts and Disaccharide Composition Analysis of Glycosaminoglycans from Rat Kidney

作　　者：王皓[1] 于广利[1] 赵峡[1] 郝翠[1] 李广生[1] 王培培[1] 

机构地区：[1]中国海洋大学医药学院山东省糖科学与糖工程重点实验室,山东青岛266003

出　　处：《光谱学与光谱分析》2010年第9期2484-2487,共4页Spectroscopy and Spectral Analysis

基　　金：国家海洋(863计划)项目(2007AA09Z445);科技部国际合作项目(2007DFA30980)资助

摘　　要：采用两步酶解和离子交换色谱从Wistar大鼠肾脏中提取糖胺聚糖,以醋酸纤维素薄膜电泳分析糖胺聚糖种类,以弱阴离子交换色谱分离各种糖胺聚糖。纯化后的糖胺聚糖分别经特定糖胺聚糖酶裂解,采用强阴离子交换高效液相色谱(SAX-HPLC)紫外检测分析其二糖组成。结果表明,Wistar大鼠肾脏糖胺聚糖主要由硫酸乙酰肝素和少量硫酸皮肤素组成。硫酸乙酰肝素含有8种二糖,其中含有乙酰基二糖总含量高达77.6%,非硫酸化二糖(Ⅳ-A)含量为59.7%;硫酸皮肤素含有6种二糖,其中单硫酸化二糖总含量为54.8%,非硫酸化二糖含量为32.9%。Glycosaminoglycans（GAGs） were extracted and purified from Wistar rat kidneys by two-step enzymatic hydrolysis and ion exchange chromatography.The sorts of GAGs were identified by electrophoresis on cellulose acetate membrane and separated on a weak anion-exchange column.Those purified GAGs were further totally hydrolyzed with specific glycosaminoglycan lyases.Their disaccharides composition and fine structures were analyzed with SAX-HPLC.The results indicated that GAGs in rat kindeys were mainly composed of heparan sulfate and small amount of dermatan sulfate.Eight kinds of disaccharides were found in heparan sulfate, and the content of acetyl-containing disaccharides was 77.6%,while nonsulfated disaccharides were 59.7%.In dermatan sulfate,six kinds of disaccharides were found.The content of monosulfated disaccharides was 49.8%,and nonsulfated disaccharides were 32.9%.

关 键 词：大鼠肾脏 糖胺聚糖 种类 二糖组成 

分 类 号：S854.43[农业科学—临床兽医学]