氢化物发生-原子荧光光谱法研究嗜热四膜虫对阿散酸饲料添加剂及其环境降解物的砷吸收  

Research on Total Arsenic Absorption of Tetrahymena Pyriformis Exposed to Arsanilic Acid Feed Additive and Its Environmental Degradation Products by HG-AFS

作　　者：韩晶晶[1] 徐芳[1] 孙群[2] 边静[1] 李玲辉[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240 [2]上海交通大学生命科学与技术学院,上海200240

出　　处：《光谱学与光谱分析》2010年第9期2567-2570,共4页Spectroscopy and Spectral Analysis

基　　金：国家"十一五"科技支撑计划项目(2006BAK10B09-09);国家自然科学基金(青年)项目(20405010);上海市科委创新行动计划项目(08142200202);上海交通大学医工交叉基金项目(YG2007MS16)资助

摘　　要：用以单细胞原生动物嗜热四膜虫对阿散酸及其降解物的砷吸收模型,研究了饲料添加剂阿散酸及其环境降解物对生物体的影响。四膜虫在含有阿散酸及其降解物的培养基中培养72 h后,洗去培养基,收集细胞。将虫体裂解,分离细胞膜和细胞内质液,经消解,用流动注射氢化物发生-原子荧光光谱法(HG-AFS)分别测定了细胞膜上和细胞膜内的砷。结果表明,阿散酸及其环境降解物可被细胞膜吸收并进入细胞内,且降解产生的无机砷比阿散酸更易被吸收,生物体通过细胞的砷吸收引起毒害。In the present paper,a model based on the arsenic absorption of the unicellular microorganism tetrahymena pyriformis from the incubation aqueous solution of feed additive arsanilic acid and its degradation products As（Ⅲ） and As（Ⅴ） was established for the investigation of the environmental impacts of feed additive arsanilic acid on the living organisms.The tetrahymena pyriformis was incubated in the incubation aqueous solution and its degradation products for 72 h,after cleaning to remove the incubation aqueous solution,the cell was broken up and separated.The cell membrane and cytoplasm were digested with nitric acid and hydrochloric acid separately.A method of arsine generation atomic fluorescence spectrometry was used for the determination of the absorbed arsenic.Experimental findings illustrate that arsenilic acid and its degradation products As（Ⅲ） and As（Ⅴ） can be absorbed by the cell membrane then enter into cytoplasm.In addition,the degradation products As（Ⅲ） and As（Ⅴ） would more easily be absorbed than the arsanilic acid.The living organisms would be poisoned via the As absorption of the cells.

关 键 词：阿散酸(Arsanilicacid) 饲料添加剂 环境释放及其降解 HG-AFS 嗜热四膜虫 生物体砷中毒 

分 类 号：O657.3[理学—分析化学]