磺酸基邻苯二甲酰亚氨甲基酞菁锌C光动力效应诱导肿瘤细胞凋亡  

Apoptosis of tumor cells induced by photodynamic therapy with sulfonated phthalimidomethyl zinc phthalocyanine C

作　　者：许建华[1] 黄自强[1] 孙建成[1] 

机构地区：[1]福建医科大学药理教研室,福州350004

出　　处：《中国现代应用药学》1999年第1期10-12,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：福建省科委资助项目!第94- 2-162号

摘　　要：目的 :研究新型光敏剂磺酸基邻苯二甲酰亚氨甲基酞菁锌C光动力效应诱导肿瘤细胞凋亡 ,探索其光动力杀伤机理。方法 :用AO/EB荧光染色法、流式细胞仪及电子显微镜观察该光敏剂光动力效应对人白血病K562细胞形态、超微结构及DNA含量的影响。结果 :药物作用 2h ,以红光照射后继续培养 3h以上 ,就可出现明显的细胞凋亡特征性改变。结论 :新型光敏剂磺酸基邻苯二甲酰亚氨甲基酞菁锌C光动力杀伤作用与诱导肿瘤细胞凋亡有关。OBJECTIVE:To observe the apoptosis of leukemia K562 cells induced by photodynamic therapy(PDT) with sulfonated phthalimidomethyl zinc phthalocyanine C(ZnPcSP-C).METHODS:Using fluorescence microscope,ransmission electron microscope and flow cytometry,the apoptosis of leukemia K562 cells induced by PDT with ZnPcSP-C was observed.RESULTS:After 2h treatment with ZnPcSP-C at 2.5～10.0μg/ml,the cells irradiated by red light and continuously cultured over 3h,the cells began to undergo typical changes of apoptosis.CONCLUSION:The antitumor effect of PDT with ZnPcSP-C may correlate to apoptosis of tumor cells.

关 键 词：酞菁锌 光动力学治疗 细胞凋亡 InPcSP-C 肿瘤 

分 类 号：R979.1[医药卫生—药品] R730.5[医药卫生—药学]