不同水平维生素A对大鼠T细胞亚群、淋巴细胞转化及细胞因子分泌的影响  

Cell Mediated Immune Functions of Rats were Modulated by Prolonged Dietary Vitamin A

作　　者：马轶凡[1] 陈荣华[1] 邓静云[1] 

机构地区：[1]南京医科大学儿科系

出　　处：《南京医科大学学报（自然科学版）》1999年第2期81-83,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：为探讨长期不同水平维生素Ａ对大鼠细胞免疫功能的影响，采用在８周内给予大鼠进食不同水平维生素Ａ的饲料，并测定其外周血Ｔ细胞亚群、淋巴细胞转化及ＩＬ－２和肿瘤坏死因子－α（ＴＮＦ－α）的分泌。结果显示：①维生素Ａ轻、重度缺乏均会导致ＣＤ４细胞减少，ＣＤ４／ＣＤ８下降，ＩＬ－２及ＴＮＦ－α分泌减少。重度维生素Ａ缺乏还导致胸腺、脾脏明显萎缩，淋巴细胞转化受抑。②维生素Ａ５倍过量组ＣＤ４细胞增多，ＣＤ４／ＣＤ８上升，淋巴细胞转化活跃，ＩＬ－２分泌增加。③维生素Ａ２５倍过量组则显示ＣＤ８细胞增多，ＣＤ４／ＣＤ８下降。结果提示，维生素Ａ缺乏或长期较大剂量的使用均可造成免疫功能的损害。Forty SD rats were divided into five groups: serious vitamin A deficiency group, marginal vitamin A deficiency group, control group, five times RDA group, twenty five times RDA group. They received experimental diets containing different amount of vitamin A for eight weeks. The results showed that: ①Both serious and marginal vitamin A deficiency led to a loss of CD4 T cell, a decreased ratio of CD4/CD8 and marked reduction in productions of IL 2 and TNF α. Moreover, serious deficiency could impair immune organs and depress the lymphocyte proliferation response. ②In five times RDA group, the number of CD4 T cells and the ratio of CD4/CD8 were higher than those of control. Besides that, five times RDA of vitamin A could stimulate the lymphocyte proliferation response and IL 2 production. ③Although no toxic symptoms were observed in twenty five times RDA group, the number of CD8 increased and the ratio of CD4/CD8 fell down. It suggested that vitamin A deficiency and twenty five times RDA of vitamin A would impair the cell mediated immunity, while five times RDA of vitamin A may benefit the cell mediated immunity during eight week using.

关 键 词：维生素A 淋巴细胞转化 T细胞亚群 IL-2 TNF 

分 类 号：R977.21[医药卫生—药品] R392.12[医药卫生—药学]