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作 者:吴晨光[1,2] 刘铨之[1,2] 何戎华[1,2] 沈捷[1,2] 柴伟栋[1,2] 崔毓桂[1,2] 陈家伟[1,2]
机构地区:[1]南京医科大学第一附属医院内分泌科 [2]镇江市第一人民医院内分泌科
出 处:《南京医科大学学报(自然科学版)》1999年第2期113-115,共3页Journal of Nanjing Medical University(Natural Sciences)
基 金:南京医科大学第一附属医院青年骨干基金
摘 要:应用细胞培养技术对新生牛主动脉平滑肌细胞进行传代培养,取第4~6代平滑肌细胞进行实验,研究不同时间、不同浓度胰岛素对平滑肌细胞前列环素(prostacyclin,PGI2)生成的影响。分别于加入胰岛素培养0、6、12、24h收集细胞上清液,并将各孔细胞计数,采用放免法测定细胞上清液中6-keto-PGF1α含量(6-keto-PGF1α是前列环素的稳定代谢产物)。结果显示:加入胰岛素培养12h与24h,胰岛素浓度与细胞上清液中6-keto-PGF1α含量呈负相关(12h:r=-0.798,P<0.001;24h:r=-0.866,P<0.001)。The bovine aortic smooth muscle cells were cultured from generation to generation. We investigated the effects of different concentrations of insulin on prostacyclin (PGI 2) production of smooth muscle cells (passage 4 ̄6) on different periods of incubation. Each well was incubated with 1 ml of culture medium containing insulin. At the end of 0 h, 6 h, 12 h, 24 h incubation, the supernatants were collected from the culture and the cells were counted in each well. The concentrations of 6 keto PGF 1α (the stable PGI 2 metabolite) in the culture medium (supernatant) were determined by a specific radioimmunoassay. The results showed that at the end of 12 h and 24 h incubation with insulin, there were significant negative correlations between insulin concentrations and 6 keto PGF 1α contents of supernatants of smooth muscle cells (12 h : r =-0.789, P <0.001; 24 h : r =-0.866, P <0.001). The results suggest that insulin may inhibit secret of prostacyclin by smooth muscle cells.
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