气相色谱外标法、内标法测定广藿香中百秋李醇含量的比较  

Comparison on Methods of Determination the Contents of Patchouli Alcohol in Herba Pogostemonis by GC-ESTD and GC-ISTD

作　　者：黄丹莹[1] 

机构地区：[1]惠州市药品检验所,广东惠州516003

出　　处：《中国药师》2010年第9期1291-1292,共2页China Pharmacist

摘　　要：目的:建立气相外标法测定广藿香中百秋李醇的含量。方法:色谱柱为弹性石英毛细管柱ZB-5(30 m×0.32 mm×0.25μm);程序升温:初始温度190℃,保持6 min,以40℃·min^(-1)的速率升温至230℃,保持2 min,进样口温度为280℃,检测器温度为280℃;分流为20:1,进样量:1μl。。结果:百秋李醇在0.011 0～0.552 5 mg·ml^(-1)的范围内呈良好线性关系(r=0.999 9);平均回收率为100.1%,RSD=2.0%(n=6)本试验所建立的外标法测得的13批样品中百秋李醇含量都高于内标法。结论:该方法准确简便、环保、重复性好,较原方法更能准确地测定广藿香中百秋李醇的含量。Objective： To establish a external standard method for determination of the patchouli alcohol in herba pogostemonis. Method： The ZB-5 column （30 m × 0. 32 mm × 0. 25μm）was used. Temperature was programmed：initial temperature was 190 ℃, and maintained around 6 minutes, then heated to 230 ℃ at rate of 40 ℃ .min^-1, maintained 2 min. The inlet temperature was 280 ℃, detector temperature was 280 ℃ ,split ratio was 20：1 ,and injection volume was 1 μl. Result： Good linearity of patchouli alcohol was within the range of 0. 011 0-0. 552 5 mg.ml^-1 （ r = 0. 999 9）. The average recovery was 100. 1% , and RSD was 2. 0% （ n = 6 ）. Conclusion： The method is simple, accurate, environmental, repeatable and more accurate than the original method of determination of patchouli alcohol in herba pogostemonis

关 键 词：气相色谱 百秋李醇 内标法 外标法 含量测定 

分 类 号：R927.2[医药卫生—药学]