一氧化氮在催乳素基础及诱发分泌中作用的生体外研究  

Study on the Role of Nitric Oxide in Basal and Induced Prolactin Secretion in Vitro

作　　者：付伟[1] 王淑芬[1] 谢启文[1] 程力岩[1] 蓝妮[1] 

机构地区：[1]中国医科大学脑研究所内分泌学研究室

出　　处：《中国医科大学学报》1999年第1期4-5,共2页Journal of China Medical University

摘　　要：目的：研究一氧化氮在催乳素基础分泌及诱发分泌中的作用。方法：原代培养大鼠腺垂体细胞，单独给予一氧化氮结合物血红蛋白（Ｈｅｍｏｇｌｏｂｉｎ，Ｈｂ）、一氧化氮合酶抑制剂Ｌ－ＮＡＭＥ（Ｌ－ｎｉｔｒｏω－ａｒｇｉｎｉｎｅｍｅｔｈｙｌｅｓｔｅｒ，）或与促甲状腺激素释放激素（ＴＲＨ）同时加入；用放射免疫分析法测定催乳素的分泌量。结果：Ｈｂ及Ｌ－ＮＡＭＥ都可以增强催乳素的基础分泌，但对ＴＲＨ引起的催乳素分泌没有显著影响。Objective:To investigate the role of nitric oxide(NO) in basal and induced prolactin(PRL) secretion. Methods:Hemoglobin(Hb,a NO scavenger),or L NAME(L nitro ω arginine methyl ester,a Nitric Oxide synthase inhibitor) was added alone or together with thyrotropin releasing hormone(TRH),to primary cell culture of rat adenohypophysis. Prolactin secreted was measured by radioimmunoassay. Results:Hb and L NAME both enhanced basal prolactin secretion.However,neither showed any significant effect on PRL secretion induced by TRH.[WT5”HZ〗Conclusion:[WT5”BZ〗NO might play a (inhibitory)regulatory role in pituitary basal prolactin secretion in vitro.

关 键 词：催乳素 一氧化氮 一氧化氮合酶 

分 类 号：R338[医药卫生—人体生理学]