4-氧和4-羟四甲基哌啶氮氧自由基及衍生物对氧自由基的清除作用  

The Scavenging Activities for O · 2 and OH·of 4 hydroxy and 4 oxy Nitroxides

作　　者：苏军华 李等松 王绪明 李文广[1,2,3,4] 吴勇杰[1,2,3,4] 田暄 

机构地区：[1]兰州军区后勤部军事医学研究所 [2]解放军兰州医学高等专科学校 [3]兰州医学院 [4]兰州大学化学系国家重点实验室

出　　处：《中国公共卫生》1999年第3期179-181,共3页Chinese Journal of Public Health

摘　　要：用Ｆｅｎｔｏｎ反应产生羟基自由基（ＯＨ·），用电子自旋捕集技术（ＥＰＲ）测定了四甲基哌啶氮氧自由基及衍生物（４－ＴＥＭＰＯ）对ＯＨ·的捕获能力。用吩嗪硫酸二甲酯（ＰＭＳ）－ＮＡＤＨ体系产生超氧阴离子自由基（Ｏ—·２），用ＮＢＴ还原法比较了４－ＴＥＭＰＯ对Ｏ—·２的清除能力。结果显示：①两个四甲基羟哌啶（ＨＴＭＰＯＨ、ＯＴＭＰＯＨ）和两个四甲基哌啶氮氧自由基（ＨＴＭＰＯ、ＯＴＭＰＯ）在４０～１６０μｍｏｌ·Ｌ－１能清除Ｏ—·２。②４－ＴＥＭＰＯ在抗氧化浓度甚至低于此浓度均使ＤＭＰＯ－ＯＨ·（二甲基吡咯啉氮氧化合物与ＯＨ·形成的加合物）的信号明显高于对照。两个阴性对照四甲基哌啶（ＨＴＭＰ。The scavenging activities for O · 2 and OH· of 4 oxy and 4 hydroxy Nitroxides and its derivatives(4 TEMPO)were studied.Superoxide anions(O · 2)generated in a phenazin methosulphate(PMS) NADH system were assayed by the reduction of mitroblue tetrazolium(NBT).Hydroxyl radicals(OH)·generated in “Fenton reaction” were detected by spin trapping technique.Nitroxide free radicals,HTMPO、OTMPO and 1 hydroxyl compounds HTMPOH、OTMPOH can scavenging O · 2 during the concentrations of 4 TEMPO are 40 160μmol·L -1 .The effect is clearly different from the control group(P<0 05 or P<0 01)·HTMP、HTMPO and 1 hydroxyl compounds can t reduce the signal of DMPO OH· during their concentrations are 1 6 160μmol·L -1 ,but the signal of DMPO OH·is raise.HTMP and OTMP have no effect for OH·and O · 2.Which indicated that the mechnnism of scavenging O · 2 may be related to the stopping radical chain reation directly or analogy with superoxide dismutase(SOD).It is necessary that the mechanisms of effect for OH·or DMPO of 4 TEMPO were studied further.

关 键 词：四甲基哌啶 氮氧自由基 氧自由基清除 TEMPO 

分 类 号：R962[医药卫生—药理学]