A novel indirect inhibitive immunoassay for sulfamethoxazole  

作　　者：Ping Ping Tang Zhao Feng Luo Ji Bao Cai Qing De Su 

机构地区：[1]Department of Chemistry, University of Science and Technology of China, Hefei 230026, China [2]Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China

出　　处：《Chinese Chemical Letters》2010年第8期955-958,共4页中国化学快报（英文版）

基　　金：supported by National Natural Science Foundation of China(No.20405013)

摘　　要：A new indirect inhibitive immunoassay using surface plasmon resonance（SPR） coupled with molecularly imprinted polymer（MIP） was developed.A sulfamethoxazole（SMX） MIP coating capillary was produced and used as an in-tube solid phase extraction（SPE） device.The MIP coating formed a nanometer film on the inner wall of the capillary.The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner.The calibration curve was generated by linear fit over the range of 0.04-10.00 ng/mL.The limit of detection was 0.01 ng/mL.This method has high sensitivity and can be performed automatically.A new indirect inhibitive immunoassay using surface plasmon resonance（SPR） coupled with molecularly imprinted polymer（MIP） was developed.A sulfamethoxazole（SMX） MIP coating capillary was produced and used as an in-tube solid phase extraction（SPE） device.The MIP coating formed a nanometer film on the inner wall of the capillary.The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner.The calibration curve was generated by linear fit over the range of 0.04-10.00 ng/mL.The limit of detection was 0.01 ng/mL.This method has high sensitivity and can be performed automatically.

关 键 词：SULFAMETHOXAZOLE Molecularly imprinted polymer Surface plasmon resonance Indirect inhibitive immunoassay 

分 类 号：TQ465.1[化学工程—制药化工] TQ533.1