Investigation of the interaction of carbofuran and DNA by voltammetry and fluorescence with the aid of chemometrics  

作　　者：Ying Xia Wang Yong Nian Ni Serge Kokot 

机构地区：[1]State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China [2]Department of Chemistry, Nanchang University, Nanchang 330031, China [3]School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, QId 4001, Australia

出　　处：《Chinese Chemical Letters》2010年第8期963-967,共5页中国化学快报（英文版）

基　　金：the financial support by the State Key Laboratory of Food Science and Technology of Nanchang University(Nos.SKLF-MB-200807 and SKLF-TS-200819)

摘　　要：The interactions of carbofuran and DNA were studied using voltammetry and fluorescence spectroscopy.The formation of carbofuran-DNA makes the current peak of DNA decreased by voltammetry method.The binding number（n） and constant（Ka） for complex carbofuran-DNA were calculated to be 1.06±0.04 and 0.11±0.03mol^-1 L,respectively by fluorescence measurement.Chemometrics approach,such as singular value decomposition（SVD） was used to evaluate the number of spectral species in the drug-DNA binding process.And the pure spectra and concentration profiles in the kinetic system were clearly deduced by multivariate curve resolution alternating least squares（MCR-ALS） with the initial estimates by evolving factor analysis（EFA）.The interactions of carbofuran and DNA were studied using voltammetry and fluorescence spectroscopy.The formation of carbofuran-DNA makes the current peak of DNA decreased by voltammetry method.The binding number（n） and constant（Ka） for complex carbofuran-DNA were calculated to be 1.06±0.04 and 0.11±0.03mol^-1 L,respectively by fluorescence measurement.Chemometrics approach,such as singular value decomposition（SVD） was used to evaluate the number of spectral species in the drug-DNA binding process.And the pure spectra and concentration profiles in the kinetic system were clearly deduced by multivariate curve resolution alternating least squares（MCR-ALS） with the initial estimates by evolving factor analysis（EFA）.

关 键 词：CARBOFURAN DNA VOLTAMMETRY CHEMOMETRICS 

分 类 号：O614.33[理学—无机化学] TG142.12[理学—化学]