DNA标记超顺磁性氧化铁粒子的磁共振增强试验  

DNA Coated Superparamagnetic Iron Oxide Particles as Contrast Agent in Magentic Resonance Imaging of Liver Tumors

作　　者：许乙凯[1] 刘杏元[1] 张嘉宁[2] 

机构地区：[1]广州第一军医大学南方医院影像中心,510515 [2]广州第一军医大学实验动物中心,510515

出　　处：《放射学实践》1999年第1期12-14,共3页Radiologic Practice

摘　　要：目的：评价ＤＮＡ标记超顺磁氧化铁粒子（ＤＮＡ－ＳＰＩＯ）对大鼠肝炎性假瘤的强化作用。方法：从猪脾中提取ＤＮＡ。在合适ＰＨ下与ＳＰＩＯ结合，制备出ＤＮＡ－ＳＰＩＯ；用弗氏完全性剂建立大鼠肝炎假瘤模型（ｎ＝８）；观察ＤＮＡ－ＳＰＩＯ对肝炎性假瘤的增强作用。结果：注射ＤＮＡ－ＳＰＩＯ（２ｍｇＦｅ／只），正常肝组织ＭＲ信号明显下降，与瘤灶的较高信号对比更加清晰。结论：ＤＮＡ－ＳＰＩＯ对提高肝脏瘤灶的早期检出水平有重要价值。Purpose: To evaluate the efficacy of a new developed MR Contrast agent, DNA coated superparamagnetic iron oxide partical (DNA-SPIO), for improving detection of liver inflammatory pseudotumors. Methods: SPIO was precipatatedfrom an ampous solution of Fe3+ and Fe2+ in an alkaline condition. DNA was expected from spleen tissue of pig. Then, DNA wascoupled to SPIO throgh PH adjustment. The DNA-SPIO was drinistered intravenously at a dose of 2mg Fe/rat into the ratmodel with liver pseudotumor induced by Freund complete adjuvant. The enhancement effects were observed with MR scans. Results: After injection of DNA-SPIO, the liver tissue showed Profoundly signal loss. In contrast, pseudotumor remained relativelyhyper-intense, on both proton density weighted and T2 weighted images. Therefore, the contact beboen the nodule and surround-ing liver tissue is markedly enhanced. Conclusion: DNA-SPIO is of high value in early detection of tumorous foci in liver.

关 键 词：DNA 造影剂 磁共振成像 DNA-SPIO 

分 类 号：R445.2[医药卫生—影像医学与核医学]