小鼠肝内胆红素尿苷二磷酸葡萄糖醛酸转移酶活性的性别差异  

Gender related differences in the activity of hepatic bilirubin UDP glucuronosyltransferase of C57BL/6J mice

作　　者：张红军[1] 祝学光[1] 叶颍江 

机构地区：[1]北京医科大学人民医院普通外科

出　　处：《北京医科大学学报》1999年第1期65-66,共2页Journal of Peking University(Health Sciences)

摘　　要：目的：探讨小鼠肝内胆红素葡萄糖醛酸化的性别差异及原因。方法：提取Ｃ５７ＢＬ／６Ｊ小鼠肝微粒体，检测胆红素尿苷二磷酸葡萄糖醛酸转移酶（ＢＵＧＴ）的活性及ＬｕｂｒｏｌＰｘ激活后活性，并以Ｎｏｒｔｈｅｒｎ印迹杂交检测肝内ＢＵＧＴ基因的表达，比较雌、雄小鼠之间的差别。结果：雄性小鼠ＢＵＧＴ活性比雌性小鼠高，激活后ＢＵＧＴ活性雄性小鼠比雌性小鼠更高，雄性小鼠肝内ＢＵＧＴ基因有更高ｍＲＮＡ水平的表达。Objective: To study the gender related differences in glucuronidation of bilirubin and its cause. Methods: Microsomes were extracted from livers of C57BL/6J mice, the activities of both native and activated bilirubin UDP glucuronosyltransferase (B UGT) with Lubrol Px in microsomes of mice were measured, and the expression of hepatic B UGT gene in both male and female mice was analysed by Northern blotting. Results: The male mice not only had higher enzymatic activities in both native and activated B UGT than that of the female mice, but also exhibited stronger expression level of hepatic B UGT gene mRNA in male mice than that in female mice. Conclusion:A significant gender related difference did exist in the glucuronidation of bilirubin in mice. The stronger expression level of hepatic B UGT gene mRNA in male mice might be responsible for the above mentioned activity differences.

关 键 词：葡萄糖醛酸 转移酶 胆红素 肝脏 性别因素 B-UGT 

分 类 号：Q555[生物学—生物化学] Q593.1