大鼠纹状体内一氧化氮合酶阳性神经元的发育  

DEVELOPMENT OF NITRIC OXIDE SYNTHASE POSITIVE NEURONS IN STRIATUM OF RAT

作　　者：张艳红 万人欣 徐慧君[2] 武义鸣[2] 秦建兵[2] 田美玲[2] 

机构地区：[1]南通师范专科学校生物系,南通226007 [2]南通医学院神经生物学研究室

出　　处：《解剖学杂志》1999年第1期35-39,共5页Chinese Journal of Anatomy

摘　　要：用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组化方法观察 E14d起至生后 2 8d这段时间大鼠纹状体一氧化氮合酶阳性神经元的发育。结果 :胚龄 16d时 ,尾壳核内已有一氧化氮合酶的表达 ,此时细胞数量少 ,细胞圆形 ,无突起或仅有一短的突起 ;胚龄 18d一氧化氮合酶阳性神经元的数量增多 ;生后 1d一氧化氮合酶阳性细胞胞体增大 ,有些突起呈点线状 ;生后 7d一氧化氮合酶阳性神经元的胞体及突起进一步发育 ,突起呈线状 ,其上出现膨体样结构 ;14d时 ,神经元进一步发育分化 ,突起变多、变长 ,出现分支 ,阳性纤维主要出现在纹状体特有的片状区内 ;2 1d时分支进一步增多 ;到生后 2 8d基本达到成体水平。一氧化氮合酶阳性神经元的分布在胚胎和生后 1d时出现于尾壳核的腹外侧 ,到生后第 7d方分布于整个尾壳核 ,但始终以腹外侧密度最大 ,且尾壳核外侧的细胞在形态上要较内侧成熟早。NADPH d histochemical method was used to investigate the development of nitric oxide synthase (NOS) neurons in the striatum of rat from embryonic day 14(E14) to postnatal day 28(P28). The results showed that NOS positive neurons appeared at the ventrolateral area of the caudate putamen (CP) at E16 day. The positive cells were round, without processes or with one or two short processes. The amount of NOS positive cells increased at E18 dya. At postnatal day one(P1), the soma size of NOS positive cells increased and their processes were stained in dotted line. At P7 days, NOS positive neurons developed contiously and their neurites extended much longer with numerous varicosities. At the P14 day some positive NOS cells continued to differentiate with multiplc dendritie arborizations and sometimes longer positive neurites were mainly located in the striatal specific potchzones. At P21 day the positive neurons became more mature and at P28 day, NOS positive cells were similar to those of the adult. NOS positive neurons were first distributed at the ventrolateral part of the striatum at E16 to P1 day and distributed all over the caudate putamen with densely from P7 to P28 day.

关 键 词：纹状体 一氧化氮合酶 神经元 发育 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学] R338[医药卫生—基础医学]