Population distributions of alele frequency of apolipoprotein E by age and gender in Han Chinese  

Population distributions of allele frequency of apolipoprotein E by age and gender in Han Chinese 1

作　　者：张建刚[1,2,3] 董秀珍 杨伟军 陆晴[1,2,3] 贺林 

机构地区：[1]中国科学院上海生命科学研究中心人类基因组计划组 [2]上海长宁区老年痴呆专科医院 [3]中国科学院上海生理研究所

出　　处：《中国药理学报》1999年第3期218-222,共5页Acta Pharmacologica Sinica

摘　　要：目的：研究在上海地区健康汉族人中不同年龄和性别的载脂蛋白Ｅ基因频率的群体分布．方法：本次调查包括６４９名健康汉族人（女：２３７人，男：４１２人）．载脂蛋白Ｅ基因用ＰＣＲ扩增，前引物：５′ＧＧＣＡＣＧＧＣＴＧＴＣＣＡＡＧＧＡＧＣＴ３′；后引物：５′ＧＡＴＧＧＣＧＣＴＧＡＧＧＣＣＧＣＧＣＴ３′．ＰＣＲ产物用５单位限制性内切酶ＣｆｏⅠ酶切，用２０％聚丙烯酰胺凝胶电泳分离．结果：ＡｐｏＥ３频率最高为８６４％，其次ＡｐｏＥ４频率为７５％，ＡｐｏＥ２频率最低为６２％．经χ２检验，等位基因及基因型频率分布符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡．结论：本项研究提供了正常中国汉族人群中载脂蛋白Ｅ三个等位基因２。AIM: To study apolipoproteins E (ApoE) allele frequency in Han Chinese based on age and gender from Shanghai metropolitan area. METHODS: Healthy Han Chinese people (F: 237 and M: 412) were involved in this study. ApoE gene was amplified by PCR using the forward primer: 5′ GGC ACG GCT GTC CAA GGA GCT 3′ and reverse primer: 5′ GAT GGC GCT GAG GCC GCG CT 3′. The PCR product was digested directly with 5 units of Cfo Ⅰ and separated by a 20 % polyacrylamide non￣dena￣turing gel. RESULTS: ApoE*3 was the commonest allele which accounted for 86 4 % of the isoforms, and ApoE*2 and ApoE*4 accounted for 6 2 % and 7 5 %, respectively. The allele and genotype frequencies were in Hardy Weinberg equilibrium by comparison with that of the corresponding theoretical distribution ( P >0 05). CONCLU￣SION: The fre￣quen￣cies of ApoE*2 , ApoE*3 , and ApoE*4 were demonstrated in the normal Chinese population.

关 键 词：载脂蛋白E 多态性 汉族 基因频率 RFLP 

分 类 号：Q513[生物学—生物化学] Q987