Velvet antler polypeptides promoted proliferation of chondrocytes and osteoblast precursors and fracture healing  

作　　者：周秋丽[1,2] 郭颖洁[1,2] 王丽娟[1,2] 王颖[1,2] 刘永强[1,2] 王岩 王本祥[1,2] 

机构地区：[1]长春中医学院新药研究中心 [2]白求恩医科大学应用基础医学研究所

出　　处：《中国药理学报》1999年第3期279-282,共4页Acta Pharmacologica Sinica

摘　　要：目的：研究鹿茸总多肽（ＶＡＴＰ）和鹿茸多肽ＶＡＰＡ，ＶＡＰＢ和ＶＡＰＣ对细胞增殖的影响．方法：分离家兔和人胚软骨细胞及鸡胚头盖骨细胞，体外加入ＶＡＴＰ，ＶＡＰＡ，ＶＡＰＢ和ＶＡＰＣ，观察其对［３Ｈ］ＴｄＲ参入细胞ＤＮＡ合成的影响．整体观察了ＶＡＴＰ促进实验性大鼠桡骨骨折愈合作用．结果：ＶＡＴＰ５０－２００ｍｇ·Ｌ－１和ＶＡＰＢ１２５－５０ｍｇ·Ｌ－１促进软骨及成骨样细胞有丝分裂，ＶＡＰＡ作用较弱，ＶＡＰＣ无效．ＶＡＴＰ１０－２０ｍｇ·ｋｇ－１加速了大鼠桡骨骨折的愈合．AIM: To study the effects of velvet antler (VA) total polypeptides (VATP) and VA polypeptides, VAP A, VAP B, and VAP C on proliferation of chondrocytes and osteoblast precusors. METH￣ODS: Chondrocytes (rabbit and human fetus) and osteoblast precusors (chick embryo) were incubated in the culture medium containing VATP or VAP A, VAP B, and VAP C. TdR incorporation into DNA was measured. Fracture healing promoting action of VATP was determined in rats. RESULTS: VATP 50-200 mg·L -1 and VAP B 12 5, 25, and 50 mg·L -1 showed most marked proliferation promoting activity for rabbit costed chondrocytes and increased incorporation of TdR from (73±9) Bq (control group) to (272±55), (327±38), and (415±32) Bq, respectively ( P <0 01). The activity of VAP A was weaker than that of VAP B, and VAP C had no activity. VATP 10 and 20 mg·kg -1 by local injection into the cross section fracture area accelerated healing of radial fracture. The healing rate of VATP treated group was higher (75 %) than that of control group ( 25 % ) ( P <0 05). CON￣CLU￣TION: VATP accelerated fracture healing by stimulating proliferation of chon￣drocytes and osteoblast precursors.

关 键 词：鹿茸 多肽 骨折愈合 软骨细胞 成骨细胞 

分 类 号：R931.74[医药卫生—生药学]