AI429618基因在小鼠胚期表达的研究  

Expression of AI429618 Gene During Mouse Embryonic Development

作　　者：陈岩[1] 赵合鑫[1] 贺洪娟[1] 刘齐[1] 张凤伟[1] 韩正滨[1] 吴琼[1] 

机构地区：[1]哈尔滨工业大学发育生物学实验室,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2010年第15期2801-2805,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30971645)

摘　　要：目的:通过对与小鼠胚胎发育相关的新基因AI429618表达模式的初步分析为揭示小鼠胚胎发育机理提供研究基础。方法:利用Northern-blot和原位杂交方法对该基因进行表达谱分析。结果:Northern结果表明该基因在E12.5,E.15.5,E18.5三个时期都有所表达,并且在E12.5的小鼠胚胎中处于一个相对较高的转录水平,E15.5表达骤降并且基本上与E18.5(略高)持平;原位杂交结果显示E9.5,E10.5的小鼠胚胎中这一基因的表达集中在端脑、中脑、后脑、腮弓、前肢芽以及尾芽,E15.5的切片原位杂交中这一基因的表达信号在胸腺,肺,肝,肾,小肠中极为显著。结论:AI429618基因在小鼠胚胎发育期有着持续广泛的表达,可能对胚胎的正常发育起着重要的调控作用。Objective：To provide a foundation for the further research by investigating the expression pattern of this novel gene.Methods：AI429618 expression patterns were characterized by Northern-blot and in situ hybridization.Results：A persistent expression was detected throughout the embryonic development period.There was the expression of the AI429618 through the 3 different stages during the embryonic periodE12.5 was highest and E18.5 was more or less the same with E15.5.The expression of the AI429618 were found in brain and branchial arches, fore-limb, tail bud by whole mount （E9.5 and E10.5） in situ hybridization analysis during earlier stage of mouse.The expression of the AI429618 in the the E15.5 section is almost ubiquitously with a high density in thymus, lung, liver, kidney and intestine.Conclusion：AI429618 may be implicated in developmental functions, particularly during earlier development stage of mouse.

关 键 词：胚胎发育 基因表达 Northern-blot 原位杂交 

分 类 号：Q95-3[生物学—动物学] Q75