正常和6-羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应  

The response of pedunculopontine nucleus neurons to muscarinic acetylcholine receptor stimulation in normal and 6-hydroxydopamine-lesioned rats

作　　者：李小颖[1] 向莉[2] 尤雪梅 惠艳娉[2] 李立博[2] 侯辰[4] 张巧俊[2] 

机构地区：[1]西安交通大学医院内科,陕西西安710061 [2]西安交通大学医学院第二附属医院脑病科,陕西西安710004 [3]陕西省第四人民医院内科,陕西西安710043 [4]西安交通大学医学院生理学与病理生理学系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2010年第5期531-535,642,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基金资助项目(SJ2008C210)~~

摘　　要：目的在体水平上观察选择性胆碱能M受体激动剂氧化震颤素(Oxo-M)对正常和6-羟基多巴胺(6-OHDA)毁损大鼠脚桥核(PPN)神经元自发电活动的影响。方法采用玻璃微电极细胞外记录和PPN内注射Oxo-M,观察PPN神经元电活动的变化。结果与正常组大鼠相比,6-OHDA毁损组大鼠PPN神经元的平均放电频率增高(P<0.01),神经元活动趋于不规则(P<0.001);PPN内注射Oxo-M对正常组和6-OHDA毁损组大鼠的PPN神经元放电频率均可产生升高、降低和无变化三种改变。然而,PPN内注射Oxo-M对正常组大鼠PPN神经元的平均放电频率无明显影响,但Oxo-M使6-OHDA毁损组大鼠PPN神经元的平均放电频率显著降低(P<0.001)。结论胆碱能M受体激动剂抑制损毁组大鼠PPN神经元的活动,其作用机制是复杂的,涉及到PPN内突触前和突触后胆碱能受体。Objective To explore the effects of oxotremorine M（Oxo-M）,muscarinic acetylcholine receptor agonist,on spontaneous firing rate of neurons of pedunculopontine nucleus（PPN） in normal and 6-hydroxydopamine（6-OHDA）-lesioned rats.Methods Change in the firing activity of PPN neurons was observed by extracellular recording in vivo and local injection of Oxo-M in the PPN.Results The results showed that the mean firing rate of PPN neurons in the lesioned rats was significantly increased compared to that in normal rats（P〈0.01）,and that the firing pattern of the neurons changed toward a more irregular firing after the lesion（P〈0.001）.Intra-PPN injection of Oxo-M induced increase,decrease or no change in the firing rate of PPN neurons in both the normal and 6-OHDA-lesioned rats.However,the mean firing rate of PPN neurons in normal rats remained unaltered by the stimulation of M receptors,but the mean firing rate of PPN neurons in the lesioned rats decreased significantly by intra-PPN injection of Oxo-M（P〈0.001）.Conclusion M receptor agonist inhibits the activity of the PPN neurons in lesioned rats;the action mechanism of the agonist is complicated,involving not only postsynaptic but also presynaptic M receptors located in different sites within the PPN.

关 键 词：脚桥核 M型胆碱能受体 氧化震颤素 帕金森病 电生理学 

分 类 号：Q426[生物学—神经生物学] R338.22[生物学—生理学]