葎草花粉致敏的小鼠肺部变应性炎症模型的建立  

Establishment of a mouse model of Humulus pollen allergen-induced allergic asthma

作　　者：李雅莉[1] 张明[2] 卢家美[1] 刘昀[1] 杨侠[1] 孙秀珍[1] 

机构地区：[1]西安交通大学医学院第二附属医院呼吸内科,陕西西安710004 [2]西安交通大学医学院第二附属医院心内科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2010年第5期562-565,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30371336)~~

摘　　要：目的建立葎草花粉粗浸液致敏激发的小鼠肺部变应性炎症模型。方法 24只BALB/c小鼠随机平均分为正常对照组和哮喘模型组。观察支气管肺泡灌洗液(BALF)中的细胞总数和嗜酸性粒细胞百分比;HE染色观察葎草花粉粗浸液激发小鼠肺部变态反应性炎症;酶联免疫吸附试验检测BALF、脾组织匀浆中的细胞因子及血清总IgE抗体。结果与正常对照组比较,模型组可诱导肺组织出现明显的变应性炎症,且病理炎症评分有统计学差异(P<0.01);BALF中的细胞总数、嗜酸性粒细胞(EOS)计数、IL-4,脾脏重量,脾组织匀浆IL-4和血清总IgE抗体均显著高于正常对照组(P<0.01)。结论葎草花粉粗浸液能够成功建立小鼠肺部变态反应性炎症模型。Objective To develop a mouse model of allergic airway inflammation by using crude Humulus pollen extract.Methods A total of 24 Balb/c mice were divided randomly into two groups： control group and asthma model group with 12 in each.After sensitization by intraperitoneal injection,the mice were challenged by intranasal instillation of crude Humulus pollen extract.The total cell number and eosinophile percentage of bronchoalveolar lavage fluid（BALF） samples were determined.The lungs of mice were stained with hematoxylin and eosin to evaluate the degree of allergic inflammation.The cytokines in BALF and spleen homogenate were assayed by enzyme-linked immunoassay（ELISA）.The total IgE in the serum was also measured by ELISA.Results Compared with the mice in normal control group,those in asthma group developed obvious allergic inflammation in the airways.Histological scoring for pulmonary inflammation significantly differed between normal group and asthma group（P〈0.01）.The total cell number,eosinophile percentage and IL-4 in BALF,spleen weight,IL-4 in spleen homogenate,and IgE antibody in serum were all significantly increased in asthma group compared with those in normal group（P〈0.01）.Conclusion Crude Humulus pollen extract can successfully induce a mouse model of allergic pulmonary inflammation.

关 键 词：葎草花粉粗浸液 哮喘 动物模型 

分 类 号：R593.1[医药卫生—内科学]