宁夏回族高脂血症患者CYP2C9＊3、MSA2756G基因多态性的研究  

CYP2C9*3 and MSA2756G gene polymorphisms in patients with hyperlipemia in Ningxia Hui population

作　　者：李居怡[1,2] 金晶 高鹏[1,2] 杜娟[1,2] 王健[3] 

机构地区：[1]宁夏医科大学基础医学院,宁夏银川750004 [2]宁夏医科大学公共卫生学院,宁夏银川750004 [3]宁夏自治区人民医院药剂科,宁夏银川750021

出　　处：《西安交通大学学报（医学版）》2010年第5期592-595,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：宁夏自然科学基金资助项目(No.NZ07109);宁夏卫生厅重点科研课题(No.W200707)~~

摘　　要：目的研究高脂血症治疗的药物相关基因CYP2C9*3、甲硫氨酸合成酶(MSA2756G)在宁夏回族高脂血症患者中的分布及其与高脂血症的关系。方法通过扩增引进限制性酶切位点(ACRS)和应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对高脂血症患者进行基因型分析。结果 180名宁夏回族高脂血症患者中,CYP2C9*3等位基因频率为3.33%,突变频率男性(3.05%)高于女性(0.28%),差异有显著性(P<0.01),而与健康对照组(3.00%)比较,差异无显著性(P>0.05);MSA2756G等位基因频率为15.83%,显著高于健康对照组(10.25%)(P<0.05),未发现性别差异。结论宁夏回族人群携带CYP2C9*3基因型的男性患高脂血症风险更大;MSA2756G可能是高脂血症的危险因子。Objective To investigate the distribution of CYP2C9＊3 and methionine synthetase（MSA2756G） genes related to drug therapy in hyperlipidemia patients of Ningxia region as well as its relation with hyperlipidemia.Methods Genotype was determined by using amplication-created restriction sites（ACRS） and polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） in hyperlipidemia patients.Results Among the 180 hyperlipidemia patients of Ningxia Hui population,the frequency of CYP2C9＊3 alleles was 3.33% and mutation rate in men（3.05%） was significantly higher than that in women（0.28%）（P〈0.01）.However,the indexes mentioned above did not differ from those of the healthy controls（3.00%）（P〈0.05）.The frequency of MSA2756G（15.83%） alleles was significantly higher than that in healthy control group（10.25%）（P〈0.05）,but there was no difference between men and women.Conclusion Men in Ningxia region who carry CYP2C9＊3 genotype have a higher risk of hyperlipidemia;MSA2756G mutation may be a risk factor for hyperlipidemia.

关 键 词：高脂血症 CYP2C9 甲硫氨酸合成酶 多态性 宁夏回族 

分 类 号：R587.1[医药卫生—内分泌]