Preproghrelin基因Leu72Met多态性与广州地区汉族人群2型糖尿病的相关性研究  

Relationships between Leu72Met polymorphism of preproghrelin gene and type 2 diabetes mellitus in Han ethnics of Guangzhou

作　　者：崔炳权[1] 何震宇[1] 林元藻[1] 

机构地区：[1]广东药学院生物化学与分子生物学系,广州510006

出　　处：《重庆医科大学学报》2010年第7期1067-1070,共4页Journal of Chongqing Medical University

基　　金：广东省医学科学技术研究基金立项项目(编号:A2009312)

摘　　要：目的:探讨Preproghrelin基因Leu72Met多态性与广州地区汉族人群2型糖尿病(Type 2 diabetes mellitus,T2DM)的相关性。方法:以广州地区汉族人群中的95位非糖尿病的正常人(对照组)和102位2型糖尿病人(T2DM组)为研究对象,采用PCR扩增目的片段、限制性内切酶BseNⅠ酶切,Native-PAGE分离和银染分析,确定各组Preproghrelin基因Leu72Met多态基因型,比较分析各组间基因型、等位基因频率分布的差异。结果:对照组和T2DM组的基因型频率分布符合Hardy-Weinberg平衡。在对照组95例样本中,CC68例、AC27例,AA0例;基因型频率分别为:0.716、0.284、0;在T2DM组102例样本中,CC71例、AC25例、AA6例,基因型频率分别为:0.696、0.245、0.059。组间基因型和等位基因频率差异无统计学意义。结论:Preproghrelin基因Leu72Met多态性与T2DM之间无显著的相关性。但在T2DM组中检测到AA基因型而对照组中未发现,提示等位基因A在T2DM发生中有某种作用,将进一步研究。Objective：To investigate the relationships between Leu72Met polymorphism ofpreproghrelin gene and type 2 diabetes mellitus （T2DM）in Han ethnics ofGuangzhou.Methods：We conducted a case-control study using 95 non-T2DM healthy individuals as controls （the control group）and 102 individuals diagnosed with T2DM as patients（the T2DM group）from populations of Han ethnics in Guangzhou,in order to detect genotypes of Leu72Met polymorphism of preproghrelin gene within each group,and to compare the differ-ences of genotypic and allelic frequencies between the two groups.Various molecular biology techniques were employed in the study in-cluding target gene segments amplification using the polymerase chain reaction（PCR）.After amplification,digestions of PCR products were carried out using restriction enzyme BseNⅠand fragments obtained were detected using Native-PAGE in conjunction with a highly sensitive silver stain method.Results：In the healthy control group,the genotypes CC and AC were detected in 68 and 27 individuals,re-spectively,while no genotype AA was detected in this group.Their corresponding genotypic frequencies were 0.716,0.284,and 0,re-spectively.In the T2DM group,the genotypes CC,AC,and AA were detected in 71,25,and 6 cases,respectively.Their corresponding genotypic frequencies were 0.696,0.245,and 0.059,respectively.Allele frequencies detected were in equilibrium with the Hardy-Wein-berg equation.However,there were no significant differences between the control group and the T2DM group in terms of genotypic and al-lelic frequencies.Conclusion：There are no significant relationships between Leu72Met polymorphism of preproghrelin gene and T2DM in Han ethnics of Guangzhou.However,genotype AA was detected in the T2DM group but not in control,which suggested that allele A may play certain roles in T2DM development.Further investigations certainly would be worth pursuing.

关 键 词：Preproghrelin基因 Leu72Met多态性 2型糖尿病(T2DM) 

分 类 号：R587.1[医药卫生—内分泌] Q754[医药卫生—内科学]