培养液NaCl浓度对杜氏盐藻3-磷酸甘油脱氢酶同工酶的影响  

Effects of NaCl Concentration in Culture Medium on Isozyme of Glycerol-3-phosphate Dehydrogenase in Dunaliella salina

作　　者：吴春蝶[1] 陈辉[2] 毛穗芳[1] 巫光宏[1] 王玉琪[1] 

机构地区：[1]华南农业大学生命科学学院,广东广州510642 [2]华南理工大学轻化工研究所,广东广州510640

出　　处：《生物技术》2010年第4期50-52,共3页Biotechnology

基　　金：华南农业大学生命科学学院2008"博士奥"科技创新项目资助

摘　　要：目的:探讨在不同NaCl浓度下,杜氏盐藻(Dunaliella salina)的3-磷酸甘油脱氢酶(Glycerol 3-phosphate dehydrogen-ase,GPDH)同工酶的活性与其渗透调节相关性。方法:采用聚丙烯酰胺凝胶电泳(polyacrylamide gel electrophoresis,PAGE)技术对在不同NaCl浓度生长的杜氏盐藻的GPDH进行同工酶电泳检测。结果:在0.5mol/LNaCl低盐的条件下,杜氏盐藻具有4种NAD+-GPDH同工酶,分别为GPDH1、GPDH2、GPDH3、GPDH4。当NaCl逐渐分别增高为2.0、3.0、4.0、5.0mol/L时,只有1种NAD+-GPDH同工酶即GPDH1。结论:GPDH1具有较高活性,这与高盐胁迫时细胞大量合成甘油进行渗透调节密切相关。Objective：To study the effect of the different NaCl concentrations on isoenzyme activity of Glycerol 3-phosphate dehydrogenase（ GPDH） that is associated with osmotic adjustment in Dunaliella salina.Method：By using polyacrylamide gel electrophoresis （ PAGE） ,we carried out the isozyme electrophoresis to detect GPDH in Dunaliella salina growing at various NaCl concentrations.Result：It showed that there were four isoenzymes of NAD＋-GPDH in Dunaliella salina growing at 0.5 mol/L NaCl,which were GPDH1,GP-DH2,GPDH3 and GPDH4.Only one isoenzyme of NAD＋-GPDH,GPDH1,was detected and shown high activity when NaCl concentration was gradually increased to 2.0,3.0,4.0 and 5.0 mol/L respectively.Conclusion：Therefore,with high activity,GPDH1 was closely related to osmotic adjustment by high-salt stress via synthesis of a large number of glycerol.

关 键 词：3-磷酸甘油脱氢酶 杜氏盐藻 同工酶 聚丙烯酰胺凝胶电泳 盐浓度 

分 类 号：Q554[生物学—生物化学]