产细菌纤维素Axy-I菌株的鉴定及产物分析  

Identification and Products Characterization of Strain Axy-I Producing Bacterial Cellulose

作　　者：施庆珊[1] 冯静[1] 冯劲[1] 疏秀林[1] 陈爱美[1] 林小平[1] 欧阳友生[1] 陈仪本[1] 

机构地区：[1]广东省微生物研究所,广东省微生物应用新技术公共实验室,广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《生物技术》2010年第4期55-58,共4页Biotechnology

基　　金：广东省科技攻关项目(2006B13001002);广东省高新技术产业化项目(2009A010700005)资助

摘　　要：目的:对1株产细菌纤维素的菌株Axy-I进行鉴定,并对其在不同培养条件下的产物进行分析。方法:通过生理生化检测和16S rDNA序列分析,对菌株Axy-I进行鉴定;比较静态培养和摇瓶动态培养7d后所产细菌纤维素的产率,并利用扫描电镜观察产物的超微观结构特征。结果:菌株Axy-I的形态、菌落特征和生理生化特性与葡糖酸醋酸杆菌属一致,16S rRNA序列长度为1436bp,与Gluconacetobacter sp.4L(Genbank登录号为:AY741144.1)的同源性达97%。静态培养细菌纤维素得率为4.72g/L,纤维直径82%分布在30-80nm之间,动态培养得率为8.12g/L,纤维直径90%分布在30～70nm之间。结论:菌株Axy-I属于葡糖酸醋酸杆菌属,动态培养所产的细菌纤维素纤维丝比静态更纤细,产率也更高。Objective：A bacterial cellulose-producing strains Axy-I was identified,and its fermentation products obtained in stationary and dynamic culture was characterized.Method：Through physiological,biochemical and 16S rDNA sequence analysis,the strain was identified; the productivity of bacterial cellulose and its ultra-micro-structural features examined by scanning electron microscopy （ SEM） in stationary culture and in shake flask agitated culture for 7 days had been compared too.Result：The strain Axy-I has the same character of the Gluconacetobacter,including morphology,colony and physiological and biochemical characteristics.The length of the 16S rDNA of the strain Axy-I is 1 436 bp,and has 97% similarity with the the Gluconacetobacter sp.4L,accession number of the Genbank is AY741144.1.The productivity of BC in stationary culture for 7 days reached 4.72g/L ,with 82% diameter distribution of BC ranges from 30 to 80 nm,and the productivity of BC in shake flask agitated culture reached 8.12 g/L,with 90% % diameter distribution of BC ranges from 30 to 70 nm.Conclusion：The strain Axy-I is a new bacterial cellulose-producing strains ,belongs to Gluconaceto-bacter.The cellosilk structure of bacterial cellulose produced in agitated culture,was more compact and fine than that produced in the static method,and the yield of bacterial cellulose was much higher too.

关 键 词：细菌纤维素 葡糖酸醋酸杆菌 培养 微结构 

分 类 号：TS721[轻工技术与工程—制浆造纸工程] Q814[生物学—生物工程]