基于小鼠模型的异基因骨髓移植中合适移植细胞量的实验研究  

A experimental study on the optimal dose of bone marrow in mice undergoing allogeneic bone marrow transplantation

作　　者：许世娟[1] 贾路[1] 徐开林[1] 丁爽[1] 陈翀[1] 曾令宇[1] 

机构地区：[1]徐州医学院移植免疫实验室,221002

出　　处：《江苏医药》2010年第15期1799-1801,共3页Jiangsu Medical Journal

摘　　要：目的探讨在小鼠异基因骨髓移植模型中的合适移植细胞量。方法 6～8周雌性BALB/c小鼠经7.0GyX线全身照射后,4h内分别输入1×106(A组)、5×106(B组)、1×107(C组)、2×107(D组)个6～8周雄性C57BL/6J小鼠的骨髓细胞,比较移植后各组小鼠存活率、外周血白细胞、T和B淋巴细胞数量及移植组的嵌合情况。结果 C组生存率明显低于其它各组(P<0.05);A、D组嵌合率达95%以上后出现回落;D组造血及免疫重建较其它组提前达正常水平。结论一定范围移植细胞量越多越有利于移植后造血及免疫重建;生存率、植入率及节约干细胞资源方面综合评估,5×106可作为移植优势剂量。Objective To study the optimal dose of bone marrow in mice undergoing allogeneic bone marrow transplantation（BMT）.Methods After treated with total body irradiation（TBI） with X-ray 7.0 Gy,the female BALB/c mice aged 6 to 8 weeks old were divided into groups of A,B,C and D according to different doses of bone marrow from male C57BL/6J mice aged 6 to 8 weeks old（1×106 ,5×106 ,1×107 and 2×107 ）,which were infused within 4 hours.Survival rates,change of peripheral blood leukocytes of all groups were observed regularly.H-2Kb cells,T and B leukomonocytes were detected by flow cytometry.Results The survival rate of mice in group C was lower than that in the other groups（P〈0.05）.Chimerism rates in groups of A and D were decreasing after chimerism rate of 95% reached.The levels of hematopoietic and immune recovery in group D recovered to the normal level earlier than those in the other groups.Conclusion Within certain range of cell numbers,the more the bone marrow cells are infused,the more are the advantages for reconstitution of hematopoiesis and immunity.On the basis of evaluating survival rates and level of engraftment,a dose of 5×106 bone marrow cells may be taken as an apropriate amount for allogeneic BMT.

关 键 词：异基因骨髓移植 

分 类 号：R457[医药卫生—治疗学]