rSj26-Sj32-HRP-IgG-Dot-ELISA用于急性日本血吸虫病患者的诊断价值  

Immunodiagnostic value of rSj26-Sj32-HR-IgG-Dot-ELISA for atients with acute Schistosomiasis jaonica

作　　者：王敏[1] 李文桂[1] 蔡世飞[1] 周必英[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国人兽共患病学报》2010年第8期758-761,共4页Chinese Journal of Zoonoses

基　　金：重庆市科委地方病重大专项基金(No.2008AB5055;2008AB5008;2008AB5054)资助

摘　　要：目的探讨rSj 26-Sj 32-HRP-IgG-Dot-ELISA用于急性日本血吸虫病患者的诊断价值。方法利用纯化的rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)建立HRP-IgG-Dot-ELISA检测急性日本血吸虫病患者血清,并以华支睾吸虫病、卫氏并殖吸虫病、棘球蚴病、乙型肝炎、肺结核病患者和健康人血清作对照,观察二者的检测结果。结果 rSj 26-Sj 32和SjAWA检测急性日本血吸虫病患者阳性检出率均为100%,特异性分别为95.35%和93.02%;二者均与华支睾吸虫病、卫氏并殖吸虫病和泡型棘球蚴病患者血清存在不同程度的交叉反应。结论纯化的rSj26-Sj32融合蛋白是一种较好的免疫诊断抗原。To study the immunodiagnostic value of rSj26-Sj32-HR-IgG-Dot-ELISA for atients with acute Schistosomiasis jaonica,sera from arients were detected by HR-IgG-Dot-ELISA which established by urified rSj26-Sj32 fusion rotein and Schistosoma jaonicum adult worm antigen（SjAWA）,and comared with detections results of sera from atients with clonorchiasis,aragonimiasis westermani,echinococciasis,heatitis B and ulmonary tuberculosis,as well as healthy eole.It showed that both of the ositive rates for rSj26-Sj32 and SjAWA in detection of sera from atients with acute schistosomiasis jaonica were 100%,and the secificities were 95.35% and 93.02%,resectively.Moreover,cross reactions different degrees were emerged between sera from atients with clonorchiasis,aragonimiasis westermani and alveolar echinococcosis and both of rSj26-Sj32 and SjAWA.It is suggested that the urified rSj26-Sj32 fusion ortein is one of the referable immunodiagnostic antigen for schistosomiasis jaonica.

关 键 词：日本血吸虫病 rSj26-Sj32融合蛋白 Dot-ELISA法 免疫诊断 

分 类 号：R383[医药卫生—医学寄生虫学]