灰飞虱对氟虫腈的抗性及其机制研究  

Fipronil resistance in small brown planthopper Laodelphax striatellus (Fallén) and mechanisms

作　　者：王耀洲[1] 韩召军[1] 

机构地区：[1]南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2010年第4期49-54,共6页Journal of Nanjing Agricultural University

基　　金：江苏省重点攻关项目(BE2006303);国家科技支撑计划项目(2006BAD02A16)

摘　　要：通过室内抗性品系筛选、现实遗传力分析、离体酶活力测定,对灰飞虱对氟虫腈抗性及其机制进行了研究。结果发现:灰飞虱对氟虫腈的抗性发展较慢,利用田间低抗种群(RR6.9倍)进行室内筛选,24代后抗性仅增加到21.2倍,其抗性现实遗传力较小,仅为0.041。利用室内筛选的氟虫腈抗性和敏感性品系进行解毒酶活力的对比研究发现:抗性灰飞虱体内的酯酶和谷胱甘肽转移酶活力与敏感品系没有显著差异,但P450单加氧酶活力是敏感品系的1.58倍。增效试验表明:在敏感品系中3种增效剂对氟虫腈均没有明显增效,但在抗性品系中,PBO对氟虫腈的增效比达到13.68,TPP和DEM的增效比仅分别为1.39和1.35。表明:灰飞虱对氟虫腈的抗性主要是由多功能氧化酶活力升高所致。In this work,fipronil resistance was selected with the experimental population in laboratory.The realized heritability （h2） of resistance and the activity of detoxification enzymes were both analyzed to declare the potentiality and mechanisms for fipronil resistance in this pest.The results showed that fipronil resistance developed slowly in small brown planthopper.The selection for 24 generations resulted only in 21.2 times of resistance.The realized heritability of the resistance was only 0.041.Comparison analysis revealed that the resistant and susceptible strain had similar activity of esterase and glutathione-S-transferase.However,the activity of cytochrome P450 monooxygenase in resistant strain was 1.58 folds higher.PBO,TPP and DEM showed no synergism on fipronil in susceptible hoppers.But in resistant hoppers PBO exhibited very high synergism （13.68 folds）,and TPP （1.39 folds） and DEM （1.35 folds） very little.These results revealed that the enhanced cytochrome P450 monooxygenase activity was the main mechanism for fipronil resistance in this pest.

关 键 词：灰飞虱 氟虫腈 抗药性 解毒酶 

分 类 号：S481.4[农业科学—农药学]