HPLC和UPLC法对比测定千里香药材中九里香酮的含量  

Comparison of UPLC and HPLC for the determination of 6,8,3',4',--tetramethoxy flavone in Murraya paniculata (L.) Jack

作　　者：王静[1] 韩正洲[1] 杨立伟[2] 徐强[1] 杨海宏[2] 

机构地区：[1]华润三九医药股份有限公司,深圳518029 [2]广东省药品检验所,广州510180

出　　处：《药物分析杂志》2010年第8期1499-1501,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:用高效液相色谱(HPLC)法与超高效液相色谱(UPLC)法对比测定千里香药材中九里香酮的含量。方法:分别建立了HPLC和UPLC测定千里香药材中九里香酮含量的方法。HPLC法为AKZONOBEL Kromasil 100-5C18(4.6mm×250mm,5μm)色谱柱,以乙腈-水(30∶70)为流动相,流速1mL.min-1,检测波长337nm,柱温30℃;UPLC法为WatersAcquityBEHC18(2.1mm×50mm,1.7μm)色谱柱,以乙腈-水(23∶77)为流动相,流速0.5mL.min-1,检测波长337nm,柱温30℃。结果:2种方法所得含量测定结果一致。HPLC法和UPLC法加样回收率的RSD分别是1.8%和1.7%。结论:HPLC和UPLC法测定千里香中九里香酮的含量,方法简便,结果准确可靠。UPLC较HPLC法更加简便迅速,既达到了分析的高通量,得到更高的分析灵敏度,又大大提高了分析速度,减少了有机溶剂的消耗。UPLC法能够成功替代HPLC法,为千里香药材质量控制提供新的检测方法。Objective：To compare the methods—HPLC and ultra performance liquid chromatography（UPLC） for the determination of 6,8,3＇,4＇,--tetramethoxy flavone in Murraya paniculata （L.） Kack.Method：Respectively establish the HPLC and UPLC methods for the determination of 6,8,3＇,4＇,--Tetramethoxy flavone in Murraya paniculata （L.） jack.A AKZONOBEL Kromasil 100-5 C18column （4.6 mm×250 mm,5 μm）was adopted with methanol-water （30：70）as mobile phase at a flow rate of 1 mL·min-1;detective wavelength was set at 337 nm and column temperature was 30 ℃ in HPLC.A Waters Acquity BEH C18 column（2.1 mm×50 mm,1.7 μm）was adopted,with acetonitrile-water （23：77）as mobile phase at a flow rate of 0.5 mL·min-1;detective wavelength was set at 337 nm and column temperature was 30 ℃ in UPLC.Results：Both of the two methods for the determination had the same results.The RSD of the average recovery of HPLC and UPLC were 1.8% respectively,1.7%.Conclusion：HPLC and UPLC methods for the determination of 6,8,3＇,4＇,--tetramethoxy flavone in Murraya paniculata （L.） Jack are simple,the results are accurate and reliable.The major advantages of UPLC,over HPLC are the speed of analysis,the narrower peaks（giving increased signal-to-noise ratio）,UPLC can not only increase separation speed and efficiency,but also reduce solvent consumption and analysis time.UPLC as a new detection method can replace HPLC for the quality control of Murraya paniculata （L.） Jack.

关 键 词：超高效液相色谱法 高效液相色谱法 九里香酮 千里香 

分 类 号：R917[医药卫生—药物分析学]