香蕉原生质体的电融合研究  

The Electrofusion of Protoplasts of Banana

作　　者：刘代[1,2] 魏岳荣[2] 胡家金[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]广东省农业科学院果树研究所,广东广州510640

出　　处：《湖南农业科学》2010年第8期128-130,共3页Hunan Agricultural Sciences

基　　金：国家科技支撑项目(2008BAD92B06);广东省科技攻关项目(2007A020200003-3);科技部国际合作项目(2009DFA31470);国家"948"项目(2008-G1)

摘　　要：以香蕉栽培种"过山香"(Musa AAB Silk cv.Guoshanxiang)和野生阿宽蕉(Musa itinerans Cheesm.AB Group)原生质体为融合试材,进行香蕉高效融合的电融合参数优化。研究表明,在交变电场(AC)200 V/cm、AC作用时间30 s、直流脉冲电压(DC)1500 V/cm、DC脉冲时间40μs、脉冲次数为2次时,融合效率最佳,融合率可达32.4%以上,原生质体活力达81.4%。In order to optimize the parameters of banana＇s electrofusion,the protoplasts that isolated from Musa AAB Silk cv.Guoshanxiang and Musa itinerans Cheesm.AB Group were taken as material of electrofusion.It was shown that when electrofusion parameters were alternate current（AC） 200 V/cm,AC duration 30 s,direct current（DC） 1 500 V/cm,DC pulse with 40 μs and 2 times,the fusion efficiency was the best,which electrofusion frequency reached over 32.4% and vitality of protoplasts reached 81.4%.

关 键 词：香蕉 原生质体 电融合 

分 类 号：S667.9[农业科学—果树学]